3. Make 176 mL of a 0.9% W/V solution of agarose

Answers

Answer 1

The agarose solution can be stored at room temperature for several weeks, provided it is kept in a clean, covered container to prevent contamination. If the solution solidifies or develops clumps, it can be melted by heating in a microwave or water bath. Therefore, you will need 2.514 g of agarose.

Agarose is a type of polysaccharide that is commonly used in molecular biology for DNA and protein electrophoresis.

To make a 176 mL of a 0.9% w/v solution of agarose, you will need to use the following steps:

Step 1: Calculate the weight of agarose needed

First, you need to calculate the weight of agarose needed for the solution.

To do this, you will use the following formula:

W = (C × V × D) / 100

where, W = weight of agarose (in grams)

C = concentration of agarose (0.9%)

V = volume of solution (176 mL)

D = density of agarose (1.5 g/mL)

Substituting the values into the formula, we get:

W = (0.9 × 176 × 1.5) / 100

W = 2.514 g

Step 2: Weigh the agarose

Use a weighing scale to measure out 2.514 g of agarose and transfer it to a clean, dry 500 mL flask.

Step 3: Add water

Add about 400 mL of distilled or deionized water to the flask. Swirl the flask gently to dissolve the agarose.

Avoid creating any air bubbles in the solution, as these can interfere with the formation of the gel.

Step 4: Make up the volume

Add more distilled or deionized water to the flask until the volume reaches 500 mL.

Swirl the flask gently to mix the solution.

Step 5: Store the agarose solution

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Related Questions

5.if 75.0 ml of 0.250 m hno3 and 75.0 ml of 0.250 m koh are mixed, what is the molarity of the salt in the resulting solution?

Answers

The molarity of the salt in the resulting solution will be 0.125 M.

What is molarity ?

When HNO3 and KOH are mixed, they react to form KNO3, a salt. The balanced equation for the reaction is:

HNO3 + KOH → KNO3 + H2O

The molarity of a solution is the number of moles of solute per liter of solution. In this case, the solute is KNO3.

The total volume of the solution is 75.0 mL + 75.0 mL = 150.0 mL.

The number of moles of KNO3 formed is:

0.250 M * 75.0 mL = 18.75 mmol

The molarity of KNO3 is:

18.75 mmol / 150.0 mL = 0.125 M

Therefore, the molarity of the salt in the resulting solution is 0.125 M.

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Describe about protecting groups in organic synthesis
(more than 4).

Answers

Protecting groups are temporary functional groups that are added to a molecule to block certain reactive sites in the molecule while allowing other reactions to proceed unhindered. These groups are used in organic synthesis to protect certain functional groups from undesired reactions and to ensure selective reactivity. They are widely used in organic synthesis to enable a specific bond to be formed without unwanted side reactions.

The protecting groups in organic synthesis are mainly divided into two types, those that protect primary alcohols and those that protect carbonyl groups. The most common protecting groups used in organic synthesis include tert-butyldimethylsilyl (TBDMS), methoxymethyl (MOM), trimethylsilyl (TMS), and tert-butoxycarbonyl (Boc).For example, the TBDMS group is commonly used to protect primary alcohols and can be removed under mild conditions using fluoride ion. The MOM group is used to protect primary alcohols and can be removed using acidic conditions. The TMS group is used to protect carbonyl groups and can be removed using fluoride ion. The Boc group is used to protect amines and can be removed using acidic conditions.

Protecting groups are essential for the synthesis of complex organic molecules, and the development of new protecting groups is an ongoing area of research in organic chemistry. In summary, protecting groups are a crucial tool in organic synthesis that help ensure the desired product is obtained and unwanted side reactions are minimized.

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The spontaneous clustering of lipids is driven by an energetically favorable increase in the _______ of water.

Answers

The spontaneous clustering of lipids is driven by an energetically favorable increase in the entropy of water.
When lipids cluster together, they create a hydrophobic environment that excludes water molecules.

This leads to an increase in the disorder or randomness of water molecules, which is known as entropy. Since the increase in entropy is energetically favorable, water molecules tend to cluster around the hydrophobic region of the lipids, causing them to spontaneously form clusters or aggregates.

This clustering helps to minimize the disruption to the water structure and maximize the stability of the system. Since the increase in entropy is energetically favorable, water molecules tend to cluster around the hydrophobic region of the lipids, causing them to spontaneously form clusters or aggregates. In summary, the energetically favorable increase in the entropy of water drives the spontaneous clustering of lipids.

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Help guys please

The diagram shows a manometer containing mercury that has a density of 1.36 × 104 kg/ m'. It is connected to a gas supply. The atmospheric pressure is 76 cm Hg.

The pressure of the gas is 1.24 × 105 Pa. Determine the value of x.

Answers

The value of x, given that the pressure of the gas is 1.24×10⁵ Pa, is 13.28 cm

How do i determine the value of x?

First, we shall determine the height. Details below:

Atmospheric pressure (Pₐ) = 76 cmHgPressure of gas (P₉) = 1.24×10⁵ Pa = 1.24×10⁵ / 133.3 = 930.23 mmHg = 930.23 / 10 = 93.023 cmHgDensity (d) = 1.36×10⁴ kg/m³ = 1.36×10⁴ / 10⁶ = 0.0136 Kg/cm³Acceleration due to gravity (g) = 9.8 m/s² = 980 cm/s²Height (h) = ?

P₉ = Pₐ + dgh

93.023 = 76 + (0.0136 × 980 × h)

93.023 = 76 + 13.328h

Collect like terms

93.023 - 76 = 13.328h

17.023 = 13.328h

Divide both sides by 13.328

h = 17.023 / 13.328

= 1.28 cm

Finally, we shall obtain the value of x by adding 12 cm as shown in the diagram to the height obtained as above. Thus, we have:

Value of x = 12 + 1.28

= 13.28 cm

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What is the pH of a 0.03M solution of Acetic Acid?
Assume for Acetic acid, Ka=1.74x10-5. Give
your answer to 2 decimal places

Answers

Therefore, the pH of a 0.03 M solution of acetic acid is 2.88. The answer should be provided in two decimal places.

Acetic acid is a weak acid, meaning it only partially dissociates in water.

To find the pH of a 0.03 M solution of acetic acid, we first need to calculate the concentration of hydrogen ions (H+) produced when the acid partially dissociates.

We can do this by using the acid dissociation constant (Ka) of acetic acid.

Ka = [H+][C2H3O2−] / [HC2H3O2]

Ka = 1.74 × 10^-5 (given)

[H+] = x[C2H3O2−]

[H+] = x[HC2H3O2]

[H+] = 0.03 - x

Substituting these values in the expression for Ka gives:

1.74 × 10^-5 = x(0.03 - x) / 0.03

Solving for x: x = 0.00132 M

[H+] = 0.00132 M

pH = -log[H+]

pH = -log(0.00132)

pH = 2.88

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Carbon will burn in sufficient oxygen to produce carbon dioxide. In an experiment, 8.40 g of carbon reacts with oxygen and 30.80 g of carbon dioxide is produced. (a) What mass of oxygen reacted with 8.40 g of carbon? (b) Calculate the percentage composition by mass of carbon dioxide.​

Answers

I'm not sure about the answer to that question, but you can try using a chemistry calculator or looking up the formula online to help you. As for the percentage composition by mass of carbon dioxide, it's 27.27% carbon and 72.73% oxygen.

Calculate the mass of nitrogen dissolved at room temperature in an 92.0 l home aquarium. assume a total pressure of 1.0 atm and a mole fraction for nitrogen of 0.78.

Answers

The mass of nitrogen dissolved in the 92.0 L home aquarium at room temperature is 778.48 grams.

The mass of gases refers to the amount of matter or substance present in a gaseous state. It represents the total mass of all the gas particles within a given volume.

In the study of gases, the mass of gases is often expressed in terms of molar mass, which is the mass of one mole of the gas. Molar mass is typically measured in grams per mole (g/mol).

The mass of gases can be calculated using the ideal gas law, which relates the pressure, volume, temperature, and number of moles of a gas. The ideal gas law equation is PV = nRT, where P is the pressure, V is the volume, n is the number of moles, R is the ideal gas constant, and T is the temperature in Kelvin.

n = X × P × V / (R × T)

Given :

P = 1.0 atm

V = 92.0 L

X = 0.78 (mole fraction of nitrogen)

T = 25 + 273.15 = 298.15 K.

n = 0.78 × 1.0 atm × 92.0 L / (0.0821 L.atm/mol.K × 298.15 K)

n = 27.86 moles

Mass of nitrogen = n × molar mass

Mass of nitrogen = 27.86 moles × 28.0 g/mol

Mass of nitrogen = 778.48 g

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Modified Lewis diagram
for each of the following.
H2
CO2
CCl4
NCl3
CH3CH2CH3
CH3SCH2CH3
NHCHCH2CH3
NH2CH2COOH
CH3OCH3
CF3CHF2
ClCH2CH2SCH2CH2Cl
(NH2)2CO
Example - H20 =Modified Lewis Diagram

Answers

A modified Lewis structure shows the electron distribution in a molecule using dashes and dots to represent the bonds and lone pairs, respectively. A molecule's Lewis structure predicts its electronic and molecular geometry. Therefore, we can use a modified Lewis structure to find the bond and electron distribution in the following compounds:H2: H:HCO2: O=C=ONCl3: NCl:NH2CH2COOH: H2N-C-COOH.

There are no lone pairs in carbon dioxide (CO2), and it has a linear shape. A carbon atom is double-bonded to each oxygen atom, resulting in two double bonds and no lone pairs of electrons. As a result, the modified Lewis structure for CO2 has no lone pairs and four bonding pairs.   Similarly, there are no lone pairs in carbon tetrachloride (CCl4), and it has a tetrahedral shape with four C-Cl bonds. Therefore, the modified Lewis structure for CCl4 has four bonding pairs and no lone pairs.  CH3CH2CH3 is propane, which has a straight-chain three-carbon molecule with a central carbon atom and three H atoms connected to each end carbon atom. There are no lone pairs of electrons in propane; thus, its modified Lewis structure has only bonding pairs.  Methanol (CH3OCH3) has a modified Lewis structure with one lone pair on the central oxygen atom and three bonding pairs, one to each hydrogen atom and one to the carbon atom.  

ClCH2CH2SCH2CH2Cl has a modified Lewis structure with one lone pair on the central sulfur atom and four bonding pairs, two to each chlorine atom and one to each carbon atom.  (NH2)2CO, or urea, is a molecule with two amino groups (NH2) and a carbonyl group (CO) in the center. It has a modified Lewis structure with two lone pairs of electrons on the oxygen atom and two nitrogen atoms with one lone pair each and two bonding pairs each.

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What best describes the result you observed when you added hcl (acid) to the artificial cytosol?

Answers

When HCl (acid) is added to the artificial cytosol, it typically results in a decrease in pH or an increase in acidity. This is because HCl is a strong acid that dissociates completely in water, releasing hydrogen ions (H+) into the solution.

The decrease in pH caused by the addition of HCl can have several effects on the artificial cytosol. It can alter the ionization states and activities of various molecules and ions present in the cytosol. For example, enzymes and proteins that are sensitive to changes in pH may undergo conformational changes or lose their functionality in an acidic environment. Additionally, the acidity may affect the solubility and stability of certain compounds in the cytosol.

The presence of hydrogen ions increases the concentration of protons in the solution, lowering the pH value. The decrease in pH may affect the chemical reactions and processes occurring in the artificial cytosol, potentially influencing the functionality and behavior of the components within the system.

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For the following reaction, 3.17 grams of hydrochloric acid are mixed with excess barium hydroxide. The reaction yields 7.02 grams of barium chloride.
hydrochloric acid (aq) + barium hydroxide (aq) barium chloride (aq) + water (l)
What is the theoretical yield of barium chloride ? grams
What is the percent yield of barium chloride ? %

Answers

The percent yield of BaCl2 is 58.8%.To find out the theoretical yield of barium chloride (BaCl2), we will use the given information to calculate the amount of barium chloride that should have been produced.

Given;3.17 grams of hydrochloric acid (HCl)7.02 grams of barium chloride (BaCl2)When we write the balanced chemical equation of the given reaction;

HCl (aq) + Ba(OH)2 (aq) → BaCl2 (aq) + 2H2O (l)

We find that for 1 mole of HCl, 1 mole of BaCl2 should be produced.

So, we can find the amount of BaCl2 that should have been produced from the amount of HCl that was used.

We can start by finding the number of moles of HCl that was used.

Molar mass of HCl = 36.5 g/mol

Moles of HCl = mass ÷ molar mass = 3.17 ÷ 36.5 = 0.087 moles

From the balanced equation, we know that 1 mole of HCl produces 1 mole of BaCl2.

Therefore, 0.087 moles of HCl should produce 0.087 moles of BaCl2.

Molar mass of BaCl2 = 137.3 g/mol

Theoretical yield of BaCl2 = moles of BaCl2 × molar mass

= 0.087 × 137.3= 11.94 grams

Therefore, the theoretical yield of BaCl2 is 11.94 grams.

Percent yield of BaCl2 is calculated using the formula;

Percent yield = (actual yield ÷ theoretical yield) × 100%

We are given that the actual yield of BaCl2 is 7.02 grams (which was obtained from the reaction).

So, Percent yield = (7.02 ÷ 11.94) × 100%

Percent yield = 58.8%.

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What is the magnitude of the charge of the electrons in 6.70 mol of neutral molecular-hydrogen gas (h2)?

Answers

The magnitude of the charge of the electrons in 6.70 moles of neutral molecular hydrogen gas, H₂ is 1.29×10⁶ C

How do i determine the magnitude of the charge of the electrons?

The following data were obtained from the question:

Number of mole (n) = 6.70 molesAvogadro's constant (N) = 6.022×10²³ Number of molecules = n × N = 6.70 × 6.022×10²³ = 4.035×10²⁴ moleculesElementary charge (C) = 1.6×10⁻¹⁹ CMagnitude of charge =?

The magnitude of the charge on the electron of the neutral hydrogen can be obtained as illustrated below:

Magnitude of charge = 2 × Number of molecules × C

= 2 × 4.035×10²⁴ × 1.6×10⁻¹⁹

= 1.29×10⁶ C

Thus, we can conclude from the above calculation that the magnitude of the charge is 1.29×10⁶ C

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Explain why each of the following changes would affect the rate of decomposition of a marble statie due to acid rain. (4 marks) CaCO 3

( s)+HNO 3

(aq)→Ca(NO 3

) 2

(aq)+CO 2

( g)+H 2

O(1) a. The concentration of the acid is increased. b. Erosion due to wind and weathering increases the surface area on the surface of the statue. c. The statue is cooled in cold winter weather. d. The partial pressure of carbon dioxide gas in the atmosphere is increased due to greenhouse gases.

Answers

This is because the reaction between the marble statue and the acid is an equilibrium reaction and the concentration of CO2 on the product side will increase as a result of increased partial pressure, which will shift the equilibrium in favor of the products.

Therefore, the rate of decomposition of the marble statue will increase if the partial pressure of CO2 gas in the atmosphere is increased.

CaCO3(s) + HNO3(aq) → Ca(NO3)2(aq) + CO2(g) + H2O(1)The reaction given above represents the decomposition of marble statue due to acid rain. The decomposition of marble can be affected by the following changes:a. The concentration of the acid is increased:

When the concentration of acid is increased, the rate of reaction between the marble statue and the acid will increase. This is because the number of acid molecules that are available to react with the marble statue would be higher.

Therefore, the rate of decomposition of the marble statue will be higher if the concentration of acid is increased.b. Erosion due to wind and weathering increases the surface area on the surface of the statue:If the erosion of the marble statue due to wind and weathering increases the surface area on the surface of the statue, then the surface area of the statue will increase. As the surface area increases, the rate of reaction between the marble statue and the acid will increase.

Therefore, the rate of decomposition of the marble statue will be higher if the surface area is increased.c. The statue is cooled in cold winter weather:If the marble statue is cooled in cold winter weather, then the rate of decomposition of the marble statue due to acid rain will decrease. This is because the reaction rate decreases with a decrease in temperature.

Therefore, the rate of decomposition of the marble statue will decrease if the statue is cooled in cold winter weather.d. The partial pressure of carbon dioxide gas in the atmosphere is increased due to greenhouse gases:

When the partial pressure of carbon dioxide gas in the atmosphere is increased due to greenhouse gases, the rate of decomposition of the marble statue due to acid rain will increase. This is because the reaction between the marble statue and the acid is an equilibrium reaction and the concentration of CO2 on the product side will increase as a result of increased partial pressure, which will shift the equilibrium in favor of the products.

Therefore, the rate of decomposition of the marble statue will increase if the partial pressure of CO2 gas in the atmosphere is increased.

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The phase of a substance depends on the relative magnitudes of the molecular _________________ and the energy of intermolecular attractions.

Answers

Molecular kinetic energy refers to the energy associated with the movement of individual molecules. The phase of a substance depends on the relative magnitudes of the molecular kinetic energy and the energy of intermolecular attractions.

The phase of a substance, whether it is a solid, liquid, or gas, is determined by the balance between two key factors: the molecular kinetic energy and the energy of intermolecular attractions.

In a substance, the higher the molecular kinetic energy, the more likely the molecules are to overcome intermolecular attractions and move freely. This results in a gaseous phase where the molecules are widely spaced and have high mobility.

The phase of a substance is determined by the interplay between molecular kinetic energy, which favors a more dispersed phase, and the energy of intermolecular attractions, which promotes a closer arrangement of molecules. The relative magnitudes of these two factors dictate whether a substance exists as a solid, liquid, or gas.

Therefore, the phase of a substance depends on the relative magnitudes of the molecular kinetic energy and the energy of intermolecular attractions.

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water molecule bond to each other via hydrogen bonds. these bonds form between the slight negative charge of , and the slight positive charge of on adjacent water molecules

Answers

Water molecular bond to each other via hydrogen bonds. These bonds form between the slight negative charge of oxygen, and the slight positive charge of hydrogen, on adjacent water molecules.

Water molecules are made up of two hydrogen atoms bonded to one oxygen atom. Oxygen is more electronegative than hydrogen, which means it attracts electrons more strongly. As a result, the oxygen atom in a water molecule carries a slight negative charge, while the hydrogen atoms carry slight positive charges. These partial charges create an electrostatic attraction between the oxygen of one water molecule and the hydrogen of another water molecule. This attraction is called a hydrogen bond.

Hydrogen bonds are relatively weak compared to covalent bonds, but they are strong enough to give water its unique properties. The hydrogen bonding between water molecules contributes to high surface tension, which allows insects to walk on water. Additionally, hydrogen bonds allow water to absorb a large amount of heat, which helps regulate temperature in organisms and stabilize Earth's climate.

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A certain liquid X has a normal boiling point of 121.70 "C and a boiling point elovation constant K i

=131 "C kg mol 1 a solution is prepared by. dissolving some olycine (C 2

H 3

NO 2

) in 800 . g of X. This solution boils at 123.7 C. Calculate the mass of C 2

H 5

NO that was dissolved. Round your answer to 2 significant digits.

Answers

The Mass of glycine = Molality × Molar mass × Mass of solvent=0.0153 × 75 × 800=91.35 g ≈ 91 g Mass of C2H5NO2 that was dissolved is approximately 91 g.

Hence, the correct option is 91.

Given,Normal boiling point of liquid X, T1

= 121.7°C Boiling point elevation constant, Kb

= 131°C kg mol-1 Mass of solvent, w2

= 800 g Boiling point of solution, T2

= 123.7°C

Now, Boiling point elevation is given by, ΔTb

= T2 - T1ΔTb

= 123.7 - 121.7ΔTb

= 2°C The boiling point elevation is given by,ΔTb

= Kb × bΔTb/Kb

= b Molality (b) of solution, b

= ΔTb/Kb

=2/131

=0.0153 mol/kg

The molality of the solution is 0.0153 mol/kg.Molar mass of glycine, C2H3NO2

= 75 g/mol

We need to calculate the mass of glycine dissolved in the solution.

The Mass of glycine

= Molality × Molar mass × Mass of solvent

=0.0153 × 75 × 800

=91.35 g ≈ 91 g Mass of C2H5NO2 that was dissolved is approximately 91 g.

Hence, the correct option is 91.

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When a 0.502 g sample of an unknown compound containing only carbon, hydrogen, and oxygen is completely burned, 1.324 gof 2

and 0.301 g of H 2

O are collected. What is the empirical formula of the compound? (Enter " 1 " if needed; blank answers receive no marks.) If the molar mass of the compound is known to be between 140 and 160 g/mol, what is the molecular formula of the compound?

Answers

The empirical formula of the compound can be determined by finding the ratio of the elements based on the given masses. In this case, the empirical formula is CH2O.

To determine the empirical formula, we calculate the moles of each element based on their masses. From the given data, we have 1.324 g of O, which corresponds to approximately 0.0828 moles. Similarly, we have 0.301 g of H, which corresponds to approximately 0.298 moles. Lastly, we have 0.502 g of the unknown compound, and by subtracting the mass of O and H, we find that the mass of C is approximately 0.118 g, which corresponds to approximately 0.0098 moles.

Next, we divide the number of moles of each element by the smallest number of moles (0.0098) to find the simplest ratio. This gives us the empirical formula CH2O, indicating that the compound contains one carbon atom, two hydrogen atoms, and one oxygen atom.

To determine the molecular formula, we need the molar mass of the compound. Since the molar mass is known to be between 140 and 160 g/mol, we calculate the molar mass of the empirical formula CH2O, which is approximately 30 g/mol. As the molar mass of the empirical formula is significantly lower than the given range, it indicates that the compound's molecular formula must be a multiple of CH2O. So that's how we can calculate the molecular formula of the compound.

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6) Write the name of each of the following polyatomic ions. a) CO 3
2

b) PO 4
2

c) C 2

H 1

d) Cr 2

O 7
2

7) Write the formula, including charge, for each of the following. a) Hydroxide b) Nitrite c) Perchlorate d) Sulfate

Answers

7) The formula including charge for Hydroxide ion, Nitrite ion, Perchlorate ion and Sulfate ion are OH−, NO2−, ClO4−, and SO42− respectively

correct question:

6) Write the name of each of the following polyatomic ions.

a) CO₃2

​b) PO 42

​c) C ₂H ₁

​d) Cr₂O ₇2

7) Write the formula, including charge, for each of the following.

a) Hydroxide

b) Nitrite

c) Perchlorate

d) Sulfate

answer:

6) Write the name of each of the following polyatomic ions.

a) CO32− (Carbonate ion)

b) PO42− (Phosphate ion)

c) C2H3O2− (Acetate ion)

d) Cr2O72− (Dichromate ion)

7) Write the formula, including charge, for each of the following.

a) Hydroxide ion - OH−

b) Nitrite ion - NO2−

c) Perchlorate ion - ClO4−

d) Sulfate ion - SO42−Polyatomic ions are made up of more than one atom.

These atoms are joined together with covalent bonds and have an overall electrical charge.

Polyatomic ions are very common in nature and they play a crucial role in various chemical processes.

Carbonate ion, Phosphate ion, Acetate ion and Dichromate ion are the names of CO32−, PO42−, C2H3O2−, and Cr2O72− respectively.

Hydroxide ion (OH−) has a charge of 1−, Nitrite ion (NO2−) has a charge of 1−,

Perchlorate ion (ClO4−) has a charge of 1−, and Sulfate ion (SO42−) has a charge of 2−.

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17. the binding of the amino acid in aminoacyl-trna is a (n) a. amide c. hemiacetal b. ester d. ether

Answers

The binding of the amino acid in aminoacyl-tRNA involves the formation of an ester bond.  Option b

Aminoacyl-tRNA is a complex molecule that plays a crucial role in protein synthesis. It consists of a tRNA molecule covalently linked to an amino acid. The amino acid is attached to the 3' end of the tRNA molecule through an ester bond.

An ester bond is formed between the carboxyl group (-COOH) of the amino acid and the hydroxyl group (-OH) of the ribose sugar at the 3' end of the tRNA molecule. This ester bond is also referred to as an ester linkage. The formation of the ester bond is catalyzed by the enzyme aminoacyl-tRNA synthetase.

The ester bond in aminoacyl-tRNA is essential for protein synthesis. During translation, the aminoacyl-tRNA molecule carries the specific amino acid to the ribosome, where it is incorporated into the growing polypeptide chain. The ester bond is later hydrolyzed, releasing the amino acid for further use in protein synthesis.

In summary, the binding of the amino acid in aminoacyl-tRNA involves the formation of an ester bond between the carboxyl group of the amino acid and the hydroxyl group of the ribose sugar in the tRNA molecule.

Option b

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A calibration curve was created to determine the quantity of protein in a solution. The calibration curve has the form of a straight line with the equation A=0.0182x+0.007 where A is the corrected absorbance of the solution and x is quantity of protein in the solution in units of micrograms ( μg ). Determine the quantity of protein in a solution that has an absorbance of 0.338. A blank solution has an absorbance of 0.055. quantity of protein:

Answers

The x = (0.283 - 0.007)/0.0182≈ 14.53 μg.

Therefore, the quantity of protein in the solution that has an absorbance of 0.338 is 14.53 μg.

The problem is to determine the quantity of protein in a solution that has an absorbance of 0.338. A blank solution has an absorbance of 0.055. The calibration curve has the form of a straight line with the equation

A=0.0182x+0.007

where A is the corrected absorbance of the solution and x is the quantity of protein in the solution in units of micrograms (μg).

To solve the problem, we need to use the equation of the calibration curve given above. But first, we need to find out the corrected absorbance of the sample solution.

The corrected absorbance is the difference between the absorbance of the sample solution and the absorbance of the blank solution.

This is given by:Corrected absorbance

= Absorbance of sample solution - Absorbance of blank solution

= 0.338 - 0.055

= 0.283 μg

Now, we can use the equation of the calibration curve to find out the quantity of protein in the solution.

This is given by:x

= (A - b)/m

Where,x

= quantity of protein in the solution A

= corrected absorbance of the sample solutionb

= intercept of the calibration curve

= 0.007m = slope of the calibration curve

= 0.0182. The x

= (0.283 - 0.007)/0.0182≈ 14.53 μg.

Therefore, the quantity of protein in the solution that has an absorbance of 0.338 is 14.53 μg.

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Calculate the percent error of the experimental melting point range of recrystallized benzoic acid and compare to the theoretical value.
Experimental melting point range: 116.3-120.8 degrees celsius
Theoretical value range: 120.0-122.0 degrees celsius (I'm not 100% sure if this value range is right for the theoretical benzoic acid)
Show work and compare please!

Answers

The percent error of the experimental melting point range of recrystallized benzoic acid and comparison with the theoretical value are:  The percent error of the experimental melting point range of recrystallized benzoic acid is 2.53% compared to the theoretical value.

Experimental melting point range: 116.3-120.8 degrees Celsius Theoretical melting point range:

120.0-122.0 degrees Celsius

To calculate percent error, the formula is:

% Error = |(Theoretical Value - Experimental Value) / Theoretical Value| * 100%Firstly,

we have to find out the Theoretical Value of benzoic acid.

Temperature at which benzoic acid melts is 122.41 °C (from literature)Here, the experimental melting point range is 116.3-120.8 degrees Celsius

Let us calculate the percent error:

% Error = |(Theoretical Value - Experimental Value) / Theoretical Value| * 100%

Theoretical Value = 122.41°C% Error = |(120.8+116.3) / 2 - 122.41| / 122.41 * 100%

% Error = 2.53%

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