4. you have been asked to generate 100 ml of the binding buffer for the affinity purification protocol you completed in lab. that buffer is composed of 100 mm tris-hcl and 150 mm nacl. what mass of each reagent is needed? briefly describe how to make the buffer. show your work

The pH values after the given volumes of acid have been added are: a) 12.18, b) 12.39, c) 11.78, d) 11.25, and e) 10.79.

To solve the problem, we need to use the balanced chemical equation for the reaction between KOH and HClO4:

KOH + HClO4 -> KClO4 + H2O

At the start of the titration, before any HClO4 has been added, we have a solution of KOH with a concentration of 0.150 M. We can use this concentration to calculate the initial concentration of hydroxide ions in the solution:

[OH-] = 0.150 M

a) Before any HClO4 has been added, the volume of the solution is 20.0 mL. At this point, no HClO4 has reacted with the KOH, so the concentration of OH- ions is still 0.150 M. To calculate the pH, we can use the formula for the dissociation constant of water:

Kw = [H+][OH-] = 1.0 x 10^-14

pH = -log[H+]

[H+] = Kw/[OH-] = 6.67 x 10^-13 M

pH = -log(6.67 x 10^-13) = 12.18

b) After 1.5 mL of HClO4 has been added, the volume of the solution is 21.5 mL. The moles of HClO4 added is:

0.125 mol/L x 0.0015 L = 1.875 x 10^-5 mol

The moles of KOH initially in the solution is:

0.150 mol/L x 0.020 L = 0.003 mol

Thus, the moles of KOH remaining after reaction with HClO4 is:

0.003 mol - 1.875 x 10^-5 mol = 0.00298125 mol

The total volume of the solution is 21.5 mL, so the new concentration of KOH is:

0.00298125 mol / 0.0215 L = 0.1387 M

Using this concentration, we can calculate the concentration of OH- ions:[OH-] = 0.1387 M

Using the same formula for Kw and pH as before, we find that:

[H+] = 4.06 x 10^-13 M

pH = -log(4.06 x 10^-13) = 12.39

c) Repeating the above process for a volume of 24.0 mL gives:

[H+] = 1.64 x 10^-12 M

pH = -log(1.64 x 10^-12) = 11.78

d) For a volume of 26.5 mL:

[H+] = 5.67 x 10^-12 M

pH = -log(5.67 x 10^-12) = 11.25

e) For a volume of 29.0 mL:

[H+] = 1.63 x 10^-11 M

pH = -log(1.63 x 10^-11) = 10.79

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The law of mass action is a fundamental principle in chemistry that relates the concentrations of reactants and products in a chemical reaction to the rate of that reaction.

It states that the rate of a chemical reaction is proportional to the product of the concentrations of the reactants, with each concentration raised to a power equal to its stoichiometric coefficient in the balanced chemical equation for the reaction.

For a chemical reaction of the form:

aA + bB ⇌ cC + dD

where A, B, C, and D are the chemical species involved in the reaction, the law of mass action can be expressed as follows:

rate = k [A]²a [B]²b

where k is the rate constant for the reaction, [A] and [B] are the concentrations of the reactants A and B, respectively, and a and b are their stoichiometric coefficients in the balanced chemical equation.

Assuming that both [A] and [B] are kept at constant concentrations, the equation reduces to:

rate = k [A]²a [B]²b = k [A]²a [B]_0²b

where [B]_0 is the initial concentration of B.

Now, let's define x as the concentration of A that has reacted (i.e., the concentration of A that has been consumed in the reaction). Since the reaction is stoichiometrically balanced, we know that the concentration of B that has reacted is b*x/a. Therefore, the concentration of A and B at any given time can be expressed as follows:

[A] = [A]_0 - x

[B] = [B]_0 - b*x/a

Substituting these expressions into the rate equation, we get:

rate = k ([A]_0 - x)²a ([B]_0 - b*x/a)²b

Expanding this expression using the binomial theorem and simplifying, we get:

rate = k [A]_0²a [B]_0²b (1 - ax/[A]_0)²(a-1) (1 - bx/[B]_0)²(b-1)

At low concentrations of x, we can approximate the terms in parentheses using their first-order Taylor series expansions:

(1 - a*x/[A]_0)²(a-1) ≈ 1 - (a-1)*x/[A]_0

(1 - b*x/[B]_0)²(b-1) ≈ 1 - (b-1)*x/[B]_0

Substituting these approximations into the rate equation, we get:

rate ≈ k [A]_0²a [B]_0²b (1 - ax/[A]_0) (1 - bx/[B]_0)

Expanding and simplifying, we get:

rate ≈ k [A]_0²a [B]_0²b - k [A]_0²a [B]_0²b (a/b)x + k [A]_0²a [B]_0²b (a/b)(a-1)/2 * x²2

This is an equation of the form:

rate = A - Bx + Cx²2

where A, B, and C are constants that depend on the reaction conditions. This equation describes a quadratic relationship between the rate of the reaction and the concentration of A that has reacted (i.e., the extent of the reaction).

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Ans) d- 1s3

The s- orbital can accommodate up to two electrons. The aforementioned configuration is faulty because there are three "s" electrons, which is not conceivable.

Since there are two electrons, 2s2 is conceivable.

The greatest number of electrons in the d orbital is 10. Thus, 5d9 is also conceivable.

The p orbital can accommodate up to 6 electrons. So 3p4 is also a possibility.

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Answer:

Explanation:

To convert the concentration of the solution from g/100 cm to g/dm^3, we need to first convert the volume unit from cm^3 to dm^3.

1 dm^3 = 10^3 cm^3

So, if 100 cm^3 of the solution contains 10 g of hydrogen peroxide, then 10^3 cm^3 (1 dm^3) of the solution contains 10 g * (10^3 / 100) = 10^3 g = 1000 g.

Therefore, the concentration of the solution in g/dm^3 is 1000 g/dm^3.

The size of a sample can affect the melting point measurement by either increasing or decreasing the observed melting point.

A larger sample size can lead to a broader melting range due to uneven heating, while a smaller sample size can result in a higher melting point due to surface effects.
The rate of heating can also affect the melting point measurement. A slow heating rate can result in a lower melting point due to partial decomposition, while a rapid heating rate can lead to a higher melting point due to kinetic factors.

The degree of chemical purity of a substance can affect the melting point measurement by increasing the observed melting point and narrowing the melting range. Impurities can lower the melting point and broaden the melting range due to eutectic effects.

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Answer:

The formula for tetraphosphorus decoxide is P4O10.

Explanation:

The density of pure iron is 7.874 g/cm³. The mass in grams of a piece of iron with a volume of 20.00 cm³ is 157.48 g.

The density of the pure iron = 7.874 g/cm³

The volume of the iron = 20.00 cm³

The expression for density is as :

Density = mass / volume

Mass = density × volume

Where

Density = 7.874 g/cm³

Volume = 20.00 cm³

Mass = 7.874 g/cm³ × 20.00 cm³

Mass = 157.48 g

Thus, the mass of the piece of the iron is 157.48 with the volume of the iron is 20.00 cm³ .

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Metals may be easily molded and bent without breaking because they are malleable and ductile.

By comparing their physical and chemical characteristics, metals and non-metals may be easily identified from one another.

Metals effectively conduct heat and electricity.

Metals often react with water and acids to create basic solutions, and they also produce positive ions in an aqueous solution, according to their chemical characteristics.

Contrarily, non-metals are neither ductile nor malleable, and they have low conductivity for both heat and electricity.

When they interact with water or acids, they can also produce neutral or acidic solutions, as well as negative ions in aqueous solutions.

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Option (b) is correct. The hardness of the mineral being tested lies under 2.5 - 3.5. This is according to the Mohs scale of hardness.

Mohs scale of hardness is used as a convenient way to help identify minerals. The hardness of the mineral is a measure of its relative resistance to scratching measured by scratching the mineral against another substance of known hardness on the Mohs Hardness Scale. This method is useful for identifying minerals in the field because you can test minerals against some very common objects such as fingernail, a penny, a nail. This scale is named from its creator, the German geologist and mineralogist Friedrich Mohs. This scale of mineral hardness is a qualitative ordinal scale from 1 to 10 that characterize scratch resistance of minerals through the ability of harder material to scratch softer material.

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The complete question is,

If we find that a mineral can leave a deep scratch in glass, what is the hardness of the mineral being tested?

(a) <2.5

(b) 2.5-3.5

(c) 3.5-4.5

(d) 4.5-5.5

(e) >5.5

Mg(OH)2's relative formula mass is 58.3.

The weighted average of the masses of the formula units on a scale where the mass of a carbon-12 atom is exactly 12 units is the relative formula mass of a substance. The "formula unit" is just the formula as it appears on paper, such as NaCl, CuSO4, 5H2O, CO2, or Cl2.

One Mg atom, two O atoms, and two H atoms make up the compound Mg(OH).

For Mg, O, and H, the atomic masses are:

Mg: 24.3

O: 16.0

H: 1.0

Mg(OH)2's relative formula mass is thus:

Relative formula mass of Mg(OH)2 = (1 x relative atomic mass of Mg) + (2 x relative atomic mass of O) + (2 x relative atomic mass of H)

= (1 x 24.3) + (2 x 16.0) + (2 x 1.0)

= 24.3 + 32.0 + 2.0

= 58.3

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Phase changes involving an increase in the degree of molecular disorder typically correspond to an increase in entropy.

Conversely, phase changes that decrease the degree of molecular disorder are associated with a decrease in entropy.
At a given temperature and pressure, the sequence of phase changes that represents an increase in the degree of molecular disorder is:
solid → liquid → gas
Therefore, the sequence of phase changes that represents a decrease in the degree of molecular disorder, and hence a decrease in entropy, is:
gas → liquid → solid
For example, when water vapor condenses to form liquid water, the degree of molecular disorder decreases, and hence entropy decreases.
in some cases, the phase change from a gas to a solid may bypass the liquid phase altogether, as in the case of deposition, where a gas transforms directly into a solid.

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The lead nitrate [tex]PB (NO_{3})_{2}[/tex] is soluble in water. It will dissociate into [tex]Pb^{2+}[/tex] (aq) ion and [tex]NO_{3}^{-}[/tex] ion.

Reaction

[tex]Pb(NO_{3})_{2} (s) + H_{2}O (l) - > Pb^{2+} (aq) + 2NO_{3}^{-} (aq)[/tex]

Lead(II) nitrate is an inorganic compound with the chemical formula Pb(NO₃)₂. It commonly occurs as a colorless crystal or white powder and, unlike most other lead(II) salts, is soluble in water.

What is lead nitrate used for?

Lead Nitrate is a white or colorless, sand-like solid. It is used in making matches and special explosives, in the dye and photography industries, and in process engraving. List because it is cited by OSHA, ACGIH, DOT, NIOSH, NTP, DEP, IARC and EPA.

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False. The distance between molecules decreases for all solids when they turn into liquids.

When a solid turns into a liquid, the distances between molecules generally decrease, rather than increase.

In a solid, molecules are typically arranged in a regular, ordered pattern and are closely packed together. As the solid is heated and melts, the molecules gain kinetic energy, causing them to vibrate and move more rapidly.

This increase in motion allows the molecules to break free of their ordered positions and slide past one another, resulting in a decrease in intermolecular distances and a transition from a solid to a liquid state.

There are some exceptions to this general trend, such as in cases where the solid structure is more porous and loosely packed than the liquid, but in most cases, the statement is false.

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Answer:

Pressure reversal

Explanation:

heat transferred into the system is equal to the work done on the system by the surroundings. If we plot an isothermal process on the xy plane, we see that as the pressure increases, the volume will decrease and as the pressure decreases, the volume will increase.

A balanced cone on its small end is in inconsistent equilibrium. Equilibrium refers to a state of balance where an object isn't accelerating or changing its stir.

An object can be in one of three types of equilibrium stable, neutral, or unstable. In stable equilibrium, an object that's displaced from its position will witness a restoring force that brings it back to its original position. For illustration, a ball at the bottom of a coliseum is in stable equilibrium because if it's displaced from its position, graveness will beget it to roll back to the bottom of the coliseum. In neutral equilibrium, an object that's displaced from its position will remain in its new position. For illustration, a ball balanced at the top of a hill is in neutral equilibrium because if it's displaced from its position, it'll remain at its new position and not roll back to the top of the hill. In unstable equilibrium, an object that's displaced from its position will witness a force that will move it further down from its original position. For illustration, a cone balanced on its small end is in unstable equilibrium because if it's displaced from its position, it'll fall over and move further down from its original position. thus, a cone balanced on its small end is in unstable equilibrium.

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A cone balanced on its small end is in stable equilibrium. In stable equilibrium, an object is in a balanced state where any slight disturbance will cause the object to return to its original position.

This is because the center of gravity of the cone is located directly above its base, creating a low center of gravity.

To understand this concept, imagine a cone standing on its small end. The base of the cone provides a wide and stable support. The center of gravity, which is the point where the weight of the cone is concentrated, is located directly above the base. This means that any slight tilt or disturbance to the cone will result in the center of gravity moving to a lower position, causing the cone to return to its original upright position.

In contrast, if the cone were balanced on its large end, it would be in an unstable equilibrium. In this case, the center of gravity is located above the narrow top of the cone, making it easy for the cone to topple over with even a slight disturbance. This is because the center of gravity is higher than the base, making the cone top-heavy.

Therefore, a cone balanced on its small end is an example of stable equilibrium.

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From the given information, 52.55 grams of ethanol are required to make a 7.1 M solution using 160.0 ml of water.

To determine how many grams of ethanol are required to make a 7.1 M solution using 160.0 ml of water, we first need to calculate the number of moles of ethanol required.

Molarity is defined as moles of solute per liter of solution, so we can use the following formula to calculate the number of moles of ethanol:

Molarity = moles of solute / volume of solution

Rearranging the formula to solve for moles of solute gives:

moles of solute = Molarity x volume of solution

Substituting the values, we get:

moles of ethanol = 7.1 mol/L x (0.160 L) = 1.136 mol

Now we can use the molecular weight of ethanol to convert moles to grams:

molecular weight of ethanol = 46.07 g/mol

mass of ethanol = moles of ethanol x molecular weight of ethanol

mass of ethanol = 1.136 mol x 46.07 g/mol

mass of ethanol = 52.55 g

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It depends on the specific cookie that you are referring to, as the percentage of total grams of carbohydrate that are sugars can vary from cookie to cookie.

The information you provided (6%, 10%, 36%, 55%) does not give enough information to determine the exact percentage of total grams of carbohydrate in the cookie that are sugars. To determine the exact percentage, you would need to consult the nutrition label or ingredient list for the specific cookie in question. The total grams of carbohydrate in a food item refers to the total amount of carbohydrates that are present in that food item. This can include various types of carbohydrates, such as sugars, starches, and fibers. The percentage of total grams of carbohydrate that are sugars specifically refers to the percentage of the total carbohydrates in the food item that are comprised of simple or complex sugars. In order to determine the exact percentage of total grams of carbohydrate in a cookie that are sugars, you would need to consult the nutrition label or ingredient list for that specific cookie. The nutrition label will typically provide information on the total grams of carbohydrate per serving, as well as the grams of sugar per serving. By dividing the grams of sugar by the total grams of carbohydrate, you can determine the percentage of total grams of carbohydrate that are sugars.

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the electron configuration for calcium can be written as:

1s2 2s2 2p6 3s2 3p6 4s2

The atomic number of calcium is 20, indicating that it has 20 electrons. The ground-state electron configuration for calcium can be written using the following rules: 1s2 2s2 2p6 3s2 3p6 4s2

The first number before each sub-shell indicates the principal quantum number (n), while the letter indicates the type of sub-shell (s, p, d, or f) and the superscript indicates the number of electrons in that sub-shell. Therefore, the electron configuration for calcium can be written as:

1s2 2s2 2p6 3s2 3p6 4s2. Electronic configuration refers to the distribution of electrons in the orbitals of an atom or ion. It describes the arrangement of electrons in the electron shells or energy levels and subshells or orbitals of an atom or ion.

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Option C. is a molecule of hydrocarbon.

Hydrocarbons are organic compounds made up of only hydrogen and carbon atoms. They are the building blocks of organic chemistry and are the main component of fossil fuels such as coal, oil, and natural gas.

Molecules of hydrocarbons can be simple or complex, and can exist as gases, liquids, or solids. Some common examples of hydrocarbon molecules include methane (CH₄), ethane (C₂H₆), propane (C₃H₈), and butane (C₄H₁₀). Alkanes, alkenes, and alkynes are all types of hydrocarbons that have different chemical and physical properties based on the arrangement of their atoms.

Therefore, the correct answer is as given above. It could then be concluded that only option C is made up of hydrogen and carbon atoms.

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The molarity of the 10.0% (by mass) aqueous solution of HCl is  [tex]3.04M[/tex]

The molarity of a 10.0% (by mass) aqueous solution of hydrochloric acid (HCl) can be calculated using the following equation:

Molarity =[tex]\frac{(mass of solute (g)) }{(molar mass of solute (g/mol) * volume of solution (L))}[/tex]

Given that the density of the solution is 1 g/mL, we can calculate the volume of the solution as follows:

Volume of solution (L) =[tex]\frac{Mass of solution (g) }{Density of solution (g/mL)}[/tex]

For a 10.0% (by mass) aqueous solution of hydrochloric acid (HCl), the molar mass of the solute is 36.46 g/mol.

Therefore, the molarity of the 10.0% (by mass) aqueous solution of HCl can be calculated as follows:

Molarity =[tex]\frac{(10 g) }{(36.46 g/mol * 0.01 L)}[/tex]

Molarity = [tex]3.04 M[/tex]

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complete question:Calculate The Molarity Of A 10.0% (By Mass) Aqueous Solution Of Hydrochloric Acid (HCI). (Density Of Solution Is 1g/ML) : a.3.04 m b.2.74 m c.0.274 m d.4.33 m

In order to produce 2.3 moles of carbon dioxide (CO₂), you would need to consume 7.9 moles of glucose. This is because in the chemical equation for respiration, glucose reacts with oxygen to form carbon dioxide and water: C6H12O6 + 6O2 → 6CO2 + 6H2O.

The by-products of the refining of petroleum are separated based on their boiling points and composition.

Petroleum refining involves the process of heating crude oil in a distillation column to separate it into its various components, which have different boiling points. The crude oil is heated and the vaporized oil rises through the column, where it condenses at different heights according to its boiling point.

Additionally, the composition of the crude oil also plays a role in the separation process. Crude oil is made up of a complex mixture of hydrocarbons, which are molecules made up of carbon and hydrogen atoms. Different types of hydrocarbons have different boiling points, so the composition of the crude oil determines the boiling points of the different components that are separated during refining.

In summary, the by-products of petroleum refining are separated based on their boiling points, which are determined by their composition of hydrocarbons.

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Faraday's law of electrolysis, which states that the quantity of substance created or consumed in an electrolytic cell is precisely proportionate to the quantity of electric charge carried through the cell, must be applied to this problem.

We use the formula: moles of substance = (electric charge / Faraday's constant), where C/mol is the charge per mole of electrons and F/mol is the Faraday constant. We must first determine how many moles of nickel were created by the specified current in order to determine the time needed to plate out 7.10 g of nickel: Electric charge is calculated as electric charge = current x time. Using the molar mass of nickel (58.69 g/mol), determine its mass: [(4.96 x t) / 96485] x 58.69] x [(moles of Ni x molar mass of Ni] = mass of Ni Calculate "t" by equating the mass of nickel produced to 7.10 g and then solving for "t": t = (7.10 x 96485) / (4.96 x 58.69) = 16.6 hours. such that the current would require 16.6 hours of application to plate out 7.10 g of nickel.

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we cannot determine the exact value of ∆G, but we can say that it decreased as a result of the increase in entropy

The Gibbs free energy change (∆G) is related to the enthalpy change (∆H) and the entropy change (∆S) through the equation:

∆G = ∆H - T∆S

where T is the temperature in Kelvin.

In this case, we know that ∆H = 10 units and ∆S = 12 units. However, we do not know the temperature, so we cannot calculate ∆G exactly.

We can, however, make some general statements about the sign and magnitude of ∆G. Since ∆H is positive (indicating an endothermic reaction) and ∆S is positive (indicating an increase in disorder), the sign of ∆G will depend on the temperature. At low temperatures, the positive ∆H term will dominate, and ∆G will be positive. At high temperatures, the negative T∆S term will dominate, and ∆G will be negative. At some intermediate temperature, ∆G will be zero, and the reaction will be at equilibrium.

Therefore, we cannot determine the exact value of ∆G, but we can say that it decreased as a result of the increase in entropy.

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For each of the gas-phase reactions given, the rate expressions are based on the appearance of each product or disappearance of each reactant.

For the reaction 2H2O(g)→2H2(g)+O2(g), the rate expressions are:

1.rate = -Δ[H2]/Δt

2.rate = -Δ[H2O]/Δt

3.rate = Δ[O2]/Δt

In this equation, the negative sign is used to denote the disappearance of the reactants, while the positive sign denotes the appearance of the products. The rate expression for this reaction shows the relationship between the change in concentration of each reactant or product to the rate of the reaction.

For the reaction 2SO2(g)+O2(g)→2SO3(g), the rate expressions are:

1.rate =  Î”[SO3]/Δt

2.rate = -Δ[SO2]/Δt

3.rate = -Δ[O2]/Δt

The negative sign is used to denote the disappearance of the reactants, while the positive sign denotes the appearance of the products. The rate expression for this reaction shows the relationship between the change in concentration of each reactant or product to the rate of the reaction.

For the reaction 2NO(g)+2H2(g)→N2(g)+2H2O(g), the rate expressions are: 1.rate =  Î”[N2]/Δt 2.rate =  Î”[H2O]/Δt 3.rate = -Δ[H2]/Δt rate = -Δ[NO]/Δt

The negative sign is used to denote the disappearance of the reactants, while the positive sign denotes the appearance of the products. The rate expression for this reaction shows the relationship between the change in concentration of each reactant or product to the

1.rate = -2 Δ[H2]/Δt

2.rate = 1 Δ[N2H4]/Δt

3.rate = -1 Δ[N2]/Δt

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147grams

Hence, The mass of one mole of magnesium nitrate$Mg{(N{O_3})_2}$ is 147grams. Note : The mass of a molecule of a substance is measured in molecular weight, which is dependent on 12 as the atomic weight of carbon-12.

The mass of one mole of magnesium nitrate is 148.3 g/mol. Then the mass of 17 moles of magnesium nitrate is 2521.1 grams.

Any substance containing 6.02 × 10²³ number of its atoms is called one mole of the substance. This number is called Avogadro number. Thus, one mole of every element contains Avogadro number of atoms. The mass of one mole of an element is called its atomic mass.

Similarly one mole of every compounds contains Avogadro number of its molecules. The mass of one mole of a compound is called its molar mass.

Molar mass of magnesium nitrate = 148.3 g/mol

then mass of 17 moles = no.of moles × molar mass.

mass = 17  × 148.3 g/mol  = 2521.2 grams.

Therefore, the mass of 17 moles of magnesium nitrate is 2521.1 grams.

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The temperature is increasing the three degrees.

The enthalpy of a reaction, also known as the heat of reaction, can be affected by changes in temperature. Enthalpy is a measure of the total energy in a system, including both heat and internal energy.

In a chemical reaction, the enthalpy change is the heat absorbed or released during the reaction, which can be measured as the temperature change of the system. When the temperature of a reaction increases, the enthalpy of the system will increase, and when the temperature decreases, the enthalpy of the system will decrease.

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The reactant within the smaller number of moles isn't always the limiting reactant.

The limiting reactant is the one that gets used up first in a chemical reaction and determines the amount of product that can be formed. The reactant that is present in the smaller number of moles may or may not be the limiting reactant, depending on its stoichiometric ratio with the other reactant(s) and the actual amounts of each reactant present in the reaction.

To determine the limiting reactant, you need to compare the stoichiometric ratios of all the reactants and calculate the amount of product that each reactant could potentially produce. The reactant that produces the least amount of product is the limiting reactant.

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