during the pcr lab, an ls23l student got lazy and decided not to put chelex inside the microcentrifuge with the student's dna. what would you expect to happen from this?

Answers

Answer 1

The DNA would be degraded by the DNAse due to the laziness of the student to not put Chelex inside the microcentrifuge.

Chelex 100 molecular-grade resins can be used to quickly and easily prepare nucleic acids from a wide range of samples, including cell, blood, and tissue cultures. DNA and RNA were prepared with Chelex 100 suitable for detection by PCR, RT-PCR, ddPCR, or LAMP.

The Chelex method of DNA extraction is suitable for the extraction of DNA from a smaller number of samples. This method is quick, and easy and does not involve any toxic organic solvents. The basic procedure involves the extraction of DNA by adding the sample to a warm suspension of Chelex at pH 10-11.

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Related Questions

what is the general term used to describe the viral component that binds to a host cell for attachment?

Answers

Viral receptors are the general term used to describe the viral component that binds to a host cell for attachment purposes.

When referring to a particular molecule or structure that aids in viral attachment on the surface of host cells, the word "receptor" should be used. The molecule(s) on the surface of the virus that binds to the receptor is referred to as "ligands." The term "viral envelope" refers to the structure that surrounds the capsid of certain viruses. All viruses use a specific type of glycoprotein to bind to viral receptors on their host cells, which are cellular molecules.

Once a virus has attached itself to and entered the host cell by breaking through the cell wall or membrane, viral replication can start. The protein covering the viral genome is removed, and the viral genome is then injected into the host cell.

 

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What is bacterial infection??

Answers

A bacterial infection occurs when bacteria enter the body, increase in number, and cause a reaction in the body. Bacteria can enter the body through an opening in your skin, such as a cut or a surgical wound, or through your airway and cause infections like bacterial pneumonia.

Answer:

Bacterial Infection is when you get infected by bacteria it can happen if you have an open wound and it was not properly cleaned or it can also happen if you are using something that has not been sanitized to pierce you skin (IT CAN GET REALLY UGLY).

Explanation:

The process of removal of wastes produced in the cells of the living organisms is called

Answers

Answer:

Excertion

Explanation:

The process of removal of waste produced in the cells of living organism is called excretion.

Answer:

excretion

Explanation:

When the cells in the living organisms perform various cellular functions, different wastes are produced. The filtration and removal of these cellular wastes from the body

what are the similarities and differences you observe between the anatomy of the sheep brain and the anatomy of the human brain? what structures were you able to visualize over the course of the dissection?

Answers

Similarities and differences between sheep and Human brain:

Similarities:

The sheep brain is similar to the human brain in having two brain stems, two optic nerves and two hemispheres.

Differences:

Compared to a sheep's brain, the human brain is larger and heavier. In comparison to the human brain, the olfactory bulb is more developed in the sheep's brain. The shape of the sheep's brain is elongated, whereas the human brain is spherical. The frontal lobe of the human brain is bigger than that of the sheep's brain. The main distinction between the human and sheep brains is that humans can use their brains to think, innovate, but sheep cannot.

Over the course of dissection structures visualizes include; cerebrum, cerebellum, brain stem, Hypothalamus, Pituitary gland, Pineal gland, Thalamus etc.

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if there were only three kinds of nucleotides, how many different types of codons would a genetic code have? (assume that the codons are three nucleotides long.)

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If there were only three kinds of nucleotides, there would be 27 different types of codons for a genetic code.

We know codons come in triplets. These triplets can be arranged in nine different ways to identify genetic codes. When multiplied, we will find that 27 different types of codons can be made from these rearrangements. This means in a genetic code, there are 27 different kinds of codons.

antibodies: question 8 options: require haptens to function make up part of the primary immune system are made from t cells are made from plasma cells none of the above

Answers

Happens are not necessary for antibodies to work. To work, it needs an antigen. They are created by T and B cells rather than T cells.

From where do happens originate?

Urushiol, a reactive Quinone-type chemical, gets oxidised in the skin cells after being ingested from a poison ivy plant, where it subsequently interacts with skin proteins to form happen adducts.

Do happens cause T cells to respond?

Low molecular weight substances that are unable to start an immune response on their own are referred to as happens. However, these tiny molecular weight substances can provide an antigenic determinant that can cause T-cell activation when they are covalently attached to circulating proteins.

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you are interested in determining how far a certain gene is from its centromere in neurospora. you find 90 asci with a first division segregation pattern and 25 asci with a second division segregation pattern. what is the map distance from gene to centromere?

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Map distance from gene to centromere is 110bp.

Genetic map distance is roughly proportional to the physical distance, i.e. the amount of DNA between two loci. Gene map distance is the distance between two points on a chromosome that are counted by the number of crossovers between them.

For example, suppose we have in Arabidopsis species  is 1.0 cM this means that this species contains approximately 150,000bp and contains approximately 50 genes in the loci . Thus, Genetic map distance is a unit for measuring distance between genes on a chromosome according to the frequency of recombination between them.

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the genetic material of the human immunodeficiency virus is rna. in hiv-infected human cells, the rna can be copied into a dna molecule by a process known as

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The RNA can be copied into a DNA molecule by a process known as Reverse transcription.

Reverse transcription is DNA dependent RNA polymerase, and it is considered the imitated enzyme of retroviruses. reverse transcriptase function to utilize only the single-stranded RNA to synthesize viral DNA.

As the name suggest it reverses the flow of genetic information from RNA to DNA  in place of DNA to RNA that is most common case in  transcription. As reverse transcriptase is important process in some retroviruses, like HIV-1. As this process requires to counter many antiretroviral therapeutics drugs .

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continental spreading centers include the_ in the us and the_ in africa

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Answer:

the answer is b

Explanation:

one cup of soft-serve ice cream contains 38 g of carbohydrate, 11 g of protein, and 18 g of fat. how many calories are there in a cup of soft-serve ice cream? one gram of carbohydrate supplies roughly 4 cal of energy. one gram of protein provides 4 cal of energy, and one gram of fat supplies 9 cal of energy.

Answers

Total calories in one cup of soft-serve ice cream that contains 38 g of carbohydrate, 11 g of protein, and 18 g of fat is 358 calories.

The nutrition facts given in the problem:

Carbohydrate supplies 4 calories per gram.

Protein supplies 4 calories per gram.

Fat supplies 9 calories per gram.

The soft-serve ice cream contains:

- 38 g of carbohydrate

- 11 g of protein

- 18 g of fat

Multiply each constituent with its energy content:

Energy from carbohydrate = 38 x 4 = 152 calories

Energy from protein = 11 x 4 = 44 calories

Energy from fat = 18 x 9 = 162 calories

Hence, energy total in the ice cream is 152 + 44 + 162 = 358 calories.

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Why would it be bad for two different populations to occupy the exact same niche at the same time of year?

Answers

Answer:

one specie's will out compete the other, forcing the other species to adapt or risk extinction

what waste product do yeast produce under anaerobic conditions? what waste product do yeast produce under anaerobic conditions? lactic acid ethyl alcohol pyruvic acid creatine

Answers

Yeast produces carbon dioxide and ethyl alcohol when it is anaerobic.

They do this through a process known as fermentation in which glucose molecules are consumed. Because the carbon dioxide produced during the baking process aids in the bread's rising, yeast is added.

What about anaerobic conditions?When the intake or loss of oxygen exceeds that of its production through photosynthesis or diffusion by physical transfer from the surrounding environment, anaerobic conditions are present. Due to the availability of organic material, microbial respiration typically consumes oxygen. A set of metabolic processes allow cells to regenerate their energy under anaerobic conditions. Fermentation is the name given to this process. The Krebs cycle is stopped when there is an anaerobic environment. Clostridium botulinum and the microorganisms that dwell close to hydrothermal vents on the deep-sea ocean floor are two examples of obligate anaerobes. Organisms that can tolerate the presence of oxygen but cannot use it for growth. Anaerobes that can develop in the absence of oxygen yet use it when it is available. When referring to plants, the terms "hypoxic" or "anaerobic conditions". When there is little to no oxygen available in the substrate or soil. Water replaces the oxygen that was present in the soil profile's pores. The microbial, fungal, and plant activities quickly deplete any remaining oxygen when saturated conditions persist.

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which movement of the head and neck would lengthen the fibers of the levator scapula on the right side of the body?

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Rotation to the left of the head and neck would lengthen the fibers of the levator scapula on the right side of the body.

The Levator Scapulae, an Axio-Appendicular muscle in the posterior triangle of the neck, attaches the upper limb to the spinal column. The sternocleidomastoid and trapezius protect the superior and inferior sides of the levator scapulae, respectively.

The levator scapulae raise the scapula and rotate it down to tilt the glenoid cavity inferiorly. If the scapula is stationary, levator scapulae contractions cause the cervical vertebral column to lateral flex to the side, stabilizing the vertebral column during rotation.

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Unlike birds and mammals, fish and reptiles grow their entire lives. What feature of their bone development is different from that of humans?

Answers

The feature of bone development of fish and reptiles which is different from that of humans is that structures such as jaw is formed from several bones being joined together while humans have only a single bone forming it.

What is a Bone?

This is referred to as the living tissues which make up the skeleton and are important as they protect and provide structural integrity to the cells of the body.

The jaw of fish and reptiles  are formed from several bones being joined together while humans have only a single bone forming this type of structure which is one of the differences.

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ecologists, and archaeologists, interested in biodiversity and animal populations, often refer to species richness in particular environments. species richness refers to

Answers

Species richness (S) is the number of species within a defined region. It's merely a numerical characteristic of an ecosystem. The species richness of a region is obtained through sampling or via a census.

Because a region is defined by the observer, species richness has been further categorized into three components to account for changes in spatial scale. They are Alpha diversity, Beta diversity and Gamma diversity.

One important thing to keep in mind is the difference between species richness and species diversity. Several factors affect small-scale species richness, including geographic factors such as the regional species pool, dispersal distance and ease of dispersal, biological factors such as competition, facilitation, and predation as well as environmental factors such as resource availability etc.

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when the amygdala jumpstarts stress-related networks peripheral to the central nervous system, what part of the body releases the chemicals such as adrenaline that produce energizing effects?

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Adrenaline is produced in the medulla by the adrenal glands as well as by some neurons in the central nervous system. During a stressful situation, adrenaline is quickly released into the bloodstream, sending impulses to organs to create a specific response.

The adrenal medulla, the innermost part of the adrenal gland, regulates the hormones that initiate the flight or fight response. The adrenal medulla secretes two main hormones: epinephrine (adrenaline) and norepinephrine (noradrenaline), which have similar functions. Adrenaline is produced by your adrenal glands and released into your bloodstream. Adrenaline, a hormone produced by your adrenal glands, serves a purpose. It assists you in dealing with dangerous situations by causing temporary changes in your body. As part of the process, your heart rate and breathing rate increase.

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Which statement best describes reproduction with two parents?
A. Smaller yeast cells grow on the side of a larger yeast cell.
B. New African violet plants can grow from leaves cut off of existing
plants.
C. Many tadpoles hatch from a cluster of eggs fertilized by cells from
a male frog.
O
D. Small sea stars can grow from arms that break off of a large sea
star.

Answers

C
eggs have to be fertilized by two parents

Similarities in what molecular structure is considered the greatest predictor of cross-reactivity with a cephalosporin in a patient with a confirmed penicillin allergy?.

Answers

Amoxicillin is the greatest predictor of cross-reactivity with a cephalosporin in a patient with a confirmed penicillin allergy.

What are penicillin and cephalosporins cross-reactivity?

Healthcare professionals often overestimate cross-reactivity linking penicillin and Cephalosporins. The device of cross-reactivity was previously believed to be related to a common β-lactam ring, but new evidence suggests it is instead related to the split of identical R-group side chains. Penicillin and cephalosporins exhibit limited and incomplete cross-reactivity of up to 7% which may be related to the 'cohort' of cephalosporin. 1 In clinical practice it is not uncommon for cephalosporins to be given to penicillin-allergic patients, especially if the history of penicillin reaction was not life.

So we can conclude that cross-reactivity between penicillin and cephalosporins occurs when the R1 side chain is identical as formerly reported.

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two 6-base sequences are present in bacterial promoters: tataat (located 10 nt upstream from the start site) and ttgaca (located 35 nt upstream from the start site). what is the significance of the fact that these two base sequences are different?

Answers

The significance of these two base sequences is that both the location of start site and the direction of transcription can be established.

What are the base sequences?

The order of the nucleotides composing the alleles within a DNA or RNA molecule is indicated by a series of bases denoted by a set of five different letters, which is known as a nucleic acid sequence. Sequences are often presented according to convention from the 5' end to the 3' end. The sense strand is used for DNA.

What are bacterial promoters?

Conserved DNA regions called bacterial promoters are crucial for the start of transcription. When we think of the classic paradigm, we typically picture promoters in intergenic areas upstream of genes enlisting RNAP holoenzyme to start transcription in one direction, leading to the production of an mRNA.

Hence the significance of these base sequences is to know the the location of start site and direction of transcription.

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what variables can affect the taste of soda

Answers

There are two major factors that affect the quality of fountain soda. First, the quality of the water mixed with CO2 will alter the taste. Second, the percentage mix of syrup to water/CO2 will affect the flavor. The mix varies in each geographical region.

How has the development of organoids helped in treating snakebites?(1 point)
A. Organoids provide toxins that help to build immunity in communities with dangerous snake populations.
B. Organoids replace stem cells in the snake that make the dangerous venom.
C. Organoids provide laboratory produced antivenin to treat a variety of snakebites.
D. Organoids help to differentiate cells in the victims of snakebites.

Answers

The development of organoids helped in treating snakebites in the sense that they provide laboratory produced antivenin to treat a variety of snakebites (Option C).

What are organoids?

The term organoids here make reference to tridimensional model structures derived from embryonic and/or adult venom snake glands which have been developed using stem cells and holds promise to be used in the future.

Therefore, with this data, we can see that the organoids can be defined as tridimensional model structures obtained from venom snake glands which are cultured as stem cells in order to obtain an outcome.

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homo erectus skull morphology includes group of answer choices thick bones and pink browridges. thin bones and small browridges. thick bones and small browridges. thick bones and large browridges.

Answers

Large browridges, a long, low, and wide base, as well as thick bones, are all characteristics of the Homo erectus cranium.

What exactly is skull morphology?

The auditory bulla of otic capsule, which is variously fused, imbedded, or connected to the skulls of vertebrates, houses the inner and middle ear systems. We can learn a lot about an animal's environmental adaptations from its morphology, or the variances for how these attachments have evolved.

Does nutrition alter skull shape?

According to the investigation, which was reported in the Proceedings of the National Academy od Sciences, those that ingested dairy and cereals had slight alterations in their skull shape. One of the main chewing muscles, the temporalis, altered position and shrank as humans migrated toward agriculture.

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A person who is homozygous for type a blood and a person who is homozygous for type b blood will have offspring with type ab blood. What relationship exists between the a and b alleles?.

Answers

The association that exists between the a and b alleles is called codominance. A genetic phenomenon known as codominance occurs when two alleles are expressed to the same degree by an organism.

What does a biological organism mean?

Living things with the capacity for reproduction, growth, and homeostasis are referred to as biological organisms. Taxonomy divides species into specific groupings, such as unicellular microorganisms like protists, bacteria, and archaea, or multicellular organisms like animals, plants, and fungus.

What two categories do organisms fall under?

Prokaryotic and eukaryotic are the two primary categories of organisms. This differentiation is based on the type of cells that make up the organism. Prokaryotic cells lack a nucleus and have a very basic structure that is indicative of their primitive nature.

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what is the most important negative effect of exposure to uv radiation in terms of reproductive success?

Answers

Exposure UV radiation have a high negative effect on reproductive success.

On live cells, ultraviolet light has several detrimental effects. A living cell exposed to UV light for an extended period of time frequently experiences changes in how the cell works as well as gene changes that result in cancer.

Sometimes, if it comes into touch with human skin, it usually results in rashes, ulcers, itching, burning, and redness.

What is reproductive success?

The number of offspring a person produces during a breeding event or lifetime is referred to as reproductive success. This is not constrained by the quantity of offspring one individual produces, but also by the ability of these offspring to reproduce.

What are UV radiations?

The sun and artificial sources, such tanning beds, both emit ultraviolet (UV) radiation, a type of non-ionizing radiation.

UV radiation overexposure can cause major health problems,

However, if it comes into touch with the skin for a long time and in high doses, skin cancer will result.

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how do ionizing radiation, ultraviolet radiation, and visible light harm microorganisms? how do microorganisms protect themselves against damage from uv and visible light?

Answers

Microorganism protect themselves against harmful UV light and visible light by using Iron minerals around themselves.

The iron minerals have unique properties in that they block damaging UV radiation while yet allowing organisms to utilise the portion of sunlight required for photosynthesis. UV rays kill cells by damaging their DNA. Two molecules of thymine, one of the bases that makes up DNA, react when exposed to light. The thymine dimer that results is extremely stable. The cell cannot perform its usual tasks if cellular processes are interfered as a result of an inadequate repair or unrepaired damage of thymine dimer.

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Microorganisms use the iron minerals in their surroundings to shield them from damaging UV and visible light.

In the presence of sunshine, some bacteria that require light may consume dissolved iron and perform photosynthesis. However, they did not emit oxygen throughout the process, unlike modern green plants. Rust and other iron minerals are waste products of the process. Because the iron minerals absorb the damaging UV radiation, organisms may still utilize the portion of sunlight required for photosynthesis. Compared to today's seas, the quantity of iron needed to create the minerals was far higher.

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In are the risks of derivatives manageable?, thomas a. Bass posits that market failures were due in part to mismanagement of derivative investments. What does he recommend?.

Answers

In "Are the risks of derivatives manageable", Thomas a. Bass posits that market failures were due in part to mismanagement of derivative investments and in which he recommends widespread regulation of derivatives or a complete ban. Hence, option A.

A securities is considered a derivative if its price is based on, or derived from, one or more underlying assets (such as interest rate, stocks, commodity, etc.). It is a contract between (at least) two parties based on the asset(s), and its value is determined by changes in the underlying asset.

Although derivatives are useful for hedging risks, they also create hazards that must be handled like any financial products. Thomas A. Bass contends that improper management of derivatives investments is the root of market failure and urges that derivatives be strictly controlled or outlawed.

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Question correction:

In are the risks of derivatives manageable?, thomas a. Bass posits that market failures were due in part to mismanagement of derivative investments. What does he recommend?

A. Bass recommends widespread regulation of derivatives or a complete ban.

B. Bass recommends further use of derivatives as a source of investment for banks and hedge funds.

C. Bass recommends a safety net for banks who fail due to their use of derivatives, citing the ethical nature of the investment.

D. Bass recommends implementing aggressive strategies to maximize returns in managing investments of wealthy private investors.

paranasal sinuses a. lighten the skull b. act as resonance chambers for sound c. produces mucus d. all of the above

Answers

paranasal sinuses are paired and symmetrical, air-filled cavities situated around the nasal cavity. the correct option is (D).

They are found in three bones of the neurocranium (braincase), the frontal bone, ethmoid bone, and sphenoid bone all of the above. The function of the paranasal sinuses is to protect the organism Which is done mostly by humidifying the air which is inhaled and facilitating the immune response of the respiratory system.

Also,  the sinuses are generally air-filled spaces that decrease the weight of the head and impact the resonance of the human voice.

The nares and anterior portion of the nasal cavities are generally lined with the mucous membranes and it contains sebaceous glands and hair follicles, this  serve to prevent the passage of large debris ( dirt) through the nasal cavity.

Types - Frontal sinuses

Ethmoidal cells (sinuses)

Maxillary sinuses

Sphenoidal sinuses

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which pre-mrna processing step is important for initiating translation? group of answer choices poly-a tail rna editing splicing 7-methylguanosine cap

Answers

The translation must start after the processing of the pre-7-methylguanosine mRNA's cap.

From yeast to mammals, the 7-methylguanosine cap is necessary for mRNA translation and cell viability. Additionally, it facilitates other RNA processing events such as splicing, poly(A) tail addition, and nuclear export in addition to playing a part in transcription elongation, mRNA stability, and degradation.

The addition of stabilizing and signaling components to the 5′ and 3′ ends of the molecule, as well as the elimination of intermediate sequences that do not specify the proper amino acids, are the three most crucial steps in pre-mRNA processing. The mRNA transcript can occasionally be "edited" after it has been transcribed.

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identify the following terms from smallest to biggest, liver ,chromosome ,white blood cell

Answers

Chromosome, white blood cell, and then liver. Hope this helps :)

in the head, postganglionic fibers leaving the cervical chain ganglia supply the regions and structures innervated by which cranial nerves?

Answers

The sympathetic chain ganglia, made up of the cervical, are the ganglia next to the sympathetic chain.

Post ganglionic: What is it?

Postganglionic fibers are the ones that run from the ganglia to the effector in the autonomous nervous system. Through metabolic modification and neurotransmitter release, post-ganglionic axons are directly in charge of alterations in the function of the target organ.

What exactly are preganglionic and postganglionic?

Acetylcholine is used by preganglionic neurons in the sympathetic nervous system as well as postganglionic neurons in the parasympathetic system (ACh). Norepinephrine and epinephrine are used by the sympathetic nervous system's postganglionic neurons. However, there are few exceptions to this, which are shown below.

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4. Combine the two equations to eliminate one of the variables, and then solve for the other. (3 points: 1 for eliminating one variable, 1 for the answer, and 1 for showing your work) Cuando la maestra encontr su telfono, vio que [blank] actualizado con ms capacidades multitareas.Qu opcin en voz pasiva completa la oracin correctamente?O lo habaO lo habanO eraO haba sido Joe and Chris share a locker at school. if they each have 8 classes and each class has 2 books, how many books are in the locker. Please show the work! :) the movement was characterized by its rejection of traditional filmmaking conventions in favor of experimentation and a spirit of iconoclasm. a) true b) false Ms.emlyn buys a hat and gloves with a coupon for 30% off her entire purchase. the gloves cost $35 before the discount. her bill before tax is $44.80.write an equation to find the original cost of the hat. Use long division to divide.(x5 + 2x12x - 5x - 12) = (x - 3) if the interest rate is zero, then $100 to be paid in 10 years has a present value that is a. less than $100. b. exactly $100. c. more than $100. d. indeterminate Ms. Lewinskys math class has k students. If p students earned a C or better on the last test, what fraction of the class did NOT eat a C or better on the last test? g the triceps muscle in the back of the upper arm extends the forearm. this muscle in a professional boxer exerts a force of 2903 n with an effective perpendicular lever arm of 2.35 cm , producing an angular acceleration of the forearm of 130.0 rad / s2 . what is the moment of inertia of the boxer's forearm? when can raw, unpackaged meat be offered for self-service? a at mongolian barbecues or sushi buffets b only when it is of the highest quality c never d when it is frozen What is the third term of the sequence 20 - n the system used to issue pairs of asymmetric encryption keys and digital certificates is called a . a. vpn b. key escrow c. pki d. tokenization or data masking Jason watched a caterpillar move 10 feet in 2 minutes. Jason says that the caterpillar's unit rate is 0.2 feet per minute, Is Json correct? Explain. How did neighboring countries influence Japanese culture? find the value of x in each case Explain the role of religion in the Ottoman and Safavid empires. How did each empire define itself religiously, and what was its relationship to the religious identity of the other empire and the outside world at large? Where relevant, also explain the attitude of the empire to religious minorities in its midst. List two important rules about the president found In the Article II of the Constitution A golf ball is thrown straight up in the air at a velocity of 8.3 . Determine the maximum height of the golf ball. (b) How long will it take the ball to reach its maximum height? (c) How long will it take the ball to fall from its maximum height to the height from which it was initially launched? where do somatic motor commands to skeletal muscles of the upper and lower appendages exit from the spinal cord? The manager of a movie theater compared ticket sales for two movies showing on the same night. For the first movie, 150 adult tickets and 100 child tickets were sold for $3,087.50 in total ticket sales. For the second movie, 200 adult tickets and 150 child tickets were sold for $4,287.50 in total ticket sales. What was the price of each adult and each child ticket?