In a typical diagnostic laboratory, reagents are pre-mixed together in master mixes that are stored in stocks in the freezer until they are needed for use. This makes testing go much more efficiently because the technician just thaws one or two solutions to mix together with the sample and the test can be run. What would happen (will happen) if a technician in the diagnostic laboratory mixed the primers together to make a master mix inside the biosafety hood that is also used for isolating RNA from patient samples?
a. the master mix is contaminated with viral RNA so all or most samples will give positive results
b. the runs will fail because the contaminating RNase in the mastermix will degrade viral RNA
c. Quality assurance will approve the runs because adheres to standard operating procedures in a diagnostic lab

Answers

Answer 1

The runs will fail because the contaminating RNase in the mastermix will degrade viral RNA if a technician in the diagnostic laboratory mixed the primers together to make a master mix inside the biosafety hood that is also used for isolating RNA from patient samples. The most likely answer is b.

Mixing the primers together inside the biosafety hood used for isolating RNA from patient samples can lead to contamination of the master mix with RNase enzymes.

RNases are enzymes that can degrade RNA, and their presence in the master mix can result in the degradation of the viral RNA present in the samples.

As a result, the runs are likely to fail as the viral RNA needed for testing would be compromised. It is crucial to maintain strict separation and prevent contamination of different laboratory processes to ensure accurate and reliable test results.

The most likely answer is b.

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The figure below is a drawing of a nucleotide. The base is the known as pyrimidine, cytosine purine, adenine pyrimidine, thymine pyrimidine, adenine pyrimidine, guanine purine, guanine

Answers

The figure shown above is a nucleotide, which is a structural component of nucleic acids such as DNA and RNA. Nucleotides are made up of a nitrogenous base, a sugar molecule, and a phosphate group.

The sugar in this nucleotide is a pentose sugar, specifically deoxyribose, which is a component of DNA.The nitrogenous base in this nucleotide is a pyrimidine, specifically thymine. The other pyrimidine base in DNA is cytosine. The purine bases in DNA are adenine and guanine.

The nucleotides in DNA are linked together by covalent bonds between the phosphate group on one nucleotide and the sugar molecule on another nucleotide. The nitrogenous bases stick out from the sugar-phosphate backbone and are paired with complementary bases on the other strand of DNA to form the double helix. In conclusion, the nucleotide shown in the figure above is made up of a deoxyribose sugar, a phosphate group, and the nitrogenous base thymine, which is a pyrimidine base consisting of a single ring.

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17) In a population of toads, 9% exhibit short tongues (recessive form) instead of long tongues (dominant form). What percentage of the population are carriers for the short tongue trait? 18) In a population of rodents, 1% exhibit purple toes (which is a recessive trait). Assuming a simple dominant-recessive relationship, determine what percentage of the population has the recessive allele.

Answers

17) Total, 2% of the population are carriers for the short tongue trait. 18) Total, 18% of the population have the recessive allele for purple toes.

To determine the percentage of the population that are carriers for the short tongue trait, we need to consider individuals who have the dominant form of the trait (long tongue) but they are heterozygous carriers of recessive allele.

Given that 9% of the population exhibit the short tongue trait (recessive form), it means that the remaining percentage (100% - 9% = 91%) represents individuals with the long tongue trait (dominant form).

Since the short tongue trait is recessive, individuals with the long tongue trait could either be homozygous dominant (LL) or heterozygous carriers (Ll) of the recessive allele.

If we assume Hardy-Weinberg equilibrium, where the population is in genetic equilibrium, the frequency of the recessive allele can be determined using the formula: q² + 2pq + p² = 1, where q represents the frequency of the recessive allele.

Since the recessive trait frequency is 9% or 0.09, we can square root this value to obtain q (recessive allele frequency):

√0.09 ≈ 0.3

Now, since p + q = 1, we can determine the frequency of the dominant allele:

1 - 0.3 = 0.7 (dominant allele frequency)

The heterozygous carrier frequency can be calculated as 2pq:

2 × 0.7 × 0.3 = 0.42

Therefore, approximately 42% of the population are carriers for the short tongue trait.

In, a simple dominant-recessive relationship, we are given that 1% of the population exhibit the recessive trait (purple toes).

Let's assume the dominant allele for normal toes is represented by "T," and the recessive allele for purple toes is represented by "t."

Given that the recessive trait frequency is 1%, or 0.01, we can equate this to the frequency of individuals who are homozygous recessive (tt).

According to the Hardy-Weinberg equilibrium formula: q² + 2pq + p² = 1, where q represents the frequency of the recessive allele:

q² = 0.01

q = √0.01 ≈ 0.1

Since p + q = 1, we can determine the frequency of the dominant allele:

p = 1 - 0.1 = 0.9

The percentage of the population with the recessive allele (carriers) can be determined by 2pq:

2 × 0.9 × 0.1 = 0.18 or 18%

Therefore, approximately 18% of the population have the recessive allele for purple toes.

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Assuming that the population is in Hardy-Weinberg equilibrium, 17). 42% of the population are carriers for the short tongue trait. 18).

17. Assuming that the population is in Hardy-Weinberg equilibrium, we can find the percentage of carriers for the short tongue trait as follows:

Let p = frequency of the dominant allele (long tongue)

q = frequency of the recessive allele (short tongue)

According to the question:

q² = 0.09 (since short tongue is recessive)⇒ q = 0.3 (taking square root of both sides)

Since p + q = 1,⇒ p = 0.7 (substituting q in above equation)

Frequency of carriers = 2pq= 2 × 0.7 × 0.3= 0.42 (or 42%)

Therefore, 42% of the population are carriers for the short tongue trait.

18. Assuming that the population is in Hardy-Weinberg equilibrium, we can find the frequency of the recessive allele as follows:

Let p = frequency of the dominant allele (for purple toes)

q = frequency of the recessive allele

According to the question:

q² = 0.01 (since purple toes is recessive)⇒ q = 0.1 (taking square root of both sides)

Since p + q = 1,⇒ p = 0.9 (substituting q in above equation)

Therefore, 10% of the population has the recessive allele.

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Briefly explain why angiosperms (flowering plants) are
the most diverse plant group.

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Angiosperms (flowering plants) are the most diverse plant group because they have adapted to a wide range of environments, including deserts, forests, grasslands, and wetlands. They have also evolved a variety of reproductive strategies, including self-pollination and cross-pollination, which has allowed them to colonize new habitats and spread rapidly.


Furthermore, angiosperms have evolved a number of mechanisms to protect their reproductive structures from herbivores and environmental stresses, such as drought and high temperatures. These include the production of toxic compounds, spines and thorns, and the ability to close their flowers in response to unfavorable conditions.

Moreover, angiosperms have also evolved a wide range of specialized structures for seed dispersal, such as fruits, nuts, and seeds with wings or barbs. This has allowed them to colonize new habitats and spread their genes over long distances, increasing their diversity and geographic range.

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Volcanic ash can be used to date fossil finds because each time it is expelled by an eruption it has the same relative proportions of radioactive isotopes. True False

Answers

Answer:

True

Explanation:

I did that question before

Trust me

Past professors may have told you that it's better to spread out your studying over days or weeks rather than cramming before the night of a big test. From a neuroscience perspective, they may be right because spreading out learning leads to inactivation of the enzyme protein phosphatase 1 activation of the enzyme protein phosphatase 1 elimination of transcription factors in hippocampal cell nuclei activation of aducunamab build-up of the Rac protein

Answers

By spreading out studying over an extended period, one can promote the inactivation of protein phosphatase 1, eliminate transcription factors in hippocampal cell nuclei, and activate aducunamab. These processes contribute to better long-term retention of information. Additionally, this approach facilitates the build-up of the Rac protein, which is important for the formation of long-term memory.

From a neuroscience perspective, it is more beneficial to spread out studying or learning over an extended period, rather than cramming all the information into the brain at once. This approach allows for better retention of information in the brain's memory system over time.

Memory formation and retention involve complex molecular and cellular changes in the brain cells. These changes require sufficient time to occur. Spreading out learning over days or weeks facilitates these processes.

One important change that takes place during the learning process is the inactivation of the enzyme protein phosphatase 1. This inactivation helps in the build-up of the Rac protein, which plays a role in the formation of long-term memory.

Furthermore, spreading out learning also leads to the elimination of transcription factors in hippocampal cell nuclei. Additionally, it activates aducunamab, an antibody that targets amyloid-beta, a protein associated with Alzheimer's disease.

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Draw a labelled diagram for the longitudinal section of
the leaf . Label the following : xylem tubes , xylem rings , phloem
tubes , parenchyma , mesophyll and epidermis.

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Phloem Tubes: This layer is located next to the xylem tubes and is responsible for transporting sugar produced during photosynthesis.Step 7: Xylem Rings: Finally, add rings of xylem cells surrounding the midrib. This layer provides additional support and helps transport nutrients up the midrib.

The leaf is an organ found in plants that is used for photosynthesis. The following is a 100-word answer on how to draw and label the longitudinal section of the leaf.Step-by-Step Guide for Longitudinal Section of a Leaf:Step 1: Draw the Leaf: First, draw the shape of the leaf and then create a line down the middle to indicate the midrib.Step 2: Epidermis: Add the outermost layer of the leaf, the epidermis. This layer includes guard cells that protect the leaf from water loss.Step 3: Mesophyll: This layer is the location where photosynthesis occurs. It consists of two types of cells, the palisade and spongy cells.Step 4: Parenchyma: This layer is made up of elongated cells that store food and water.Step 5: Xylem Tubes: This layer is located near the bottom of the leaf and is responsible for transporting water and nutrients from the roots to the other parts of the plant. Phloem Tubes: This layer is located next to the xylem tubes and is responsible for transporting sugar produced during photosynthesis.Step 7: Xylem Rings: Finally, add rings of xylem cells surrounding the midrib. This layer provides additional support and helps transport nutrients up the midrib.

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what is the purpose of the cartilaginous rings that surround the trachea?

Answers

The purpose of the cartilaginous rings that surround the trachea is to provide structural support and prevent the collapse of the trachea during breathing. The trachea is a flexible tube that allows the passage of air between the larynx and the bronchi in the respiratory system.

The rings, which are C-shaped incomplete rings of cartilage, are positioned on the anterior and lateral sides of the trachea.

These cartilaginous rings give rigidity to the trachea and help maintain its open shape, ensuring that the airway remains patent even when there is a decrease in pressure during inhalation. The C-shape of the rings allows flexibility and accommodation of movement, such as swallowing or neck extension, without obstructing the tracheal lumen.

The presence of cartilaginous rings in the trachea is essential for proper airflow and protection of the airway. Without these rings, the trachea would be prone to collapse or obstruction, leading to breathing difficulties and respiratory problems.

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During the luteal phase of the menstrual cycle there is a large amount of progesterone being produced. True False QUESTION 4 Estrogen is at its highest levels at the end of the luteal phase of the mentrual cycle. True False QUESTION 5 The corpus albicans produces progesterone. True False QUESTION 6 The progesterone produced during the mentrual cycle has a postive feedback effect on GnRH levels and thus increases GnRH levels. True False

Answers

QUESTION 1During the luteal phase of the menstrual cycle, there is a large amount of progesterone being produced. TrueQUESTION 2False. Estrogen is at its highest levels at the end of the follicular phase of the menstrual cycle.QUESTION 3False. The corpus luteum produces progesterone.QUESTION 4False.

The progesterone produced during the menstrual cycle has a negative feedback effect on GnRH levels and thus decreases GnRH levels.The luteal phase of the menstrual cycle is the second phase of the menstrual cycle. It occurs after ovulation when the ruptured follicle forms the corpus luteum.

The corpus luteum is a temporary endocrine gland that secretes estrogen and progesterone, preparing the uterus for the implantation of the fertilized egg.The luteal phase lasts around 14 days and ends with the onset of menstruation. During this phase, the corpus luteum produces a large amount of progesterone, which causes the lining of the uterus to thicken and become more vascularized. Progesterone also helps to prepare the mammary glands for lactation in case pregnancy occurs.

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What is anatomy? explain the experience anatomy in detail
What is personalized mobile marketing, according to your experience does the personalized mobile marketing increases the sales/ purchase. Explain its advantages and disadvantages
Discuss the classes of Mobile Commerce Application with suitable example.
Describe the stages involved in purchase decision. Also explain all the roles played at each stage

Answers

1. Anatomy is the study of the structure of living organisms.

2. Experience in anatomy involves hands-on learning and active engagement in studying anatomy.

3. Personalized mobile marketing delivers tailored content to increase sales, but privacy concerns and information overload can be disadvantages.

4. Classes of mobile commerce applications include mobile banking, shopping, ticketing, payment, and advertising.

5. The purchase decision process includes problem recognition, information search, evaluation of alternatives, purchase decision, and post-purchase evaluation, with roles played by marketers, information sources, retailers, and customer support.

1. Anatomy is the branch of science that deals with the structure and organization of living organisms. It involves the study of tissues, cells, and their relationships with each other.

2. Experience anatomy refers to a hands-on or immersive approach to studying anatomy, where individuals actively engage in activities and observations to enhance their understanding of the subject. It can involve the dissection of cadavers, the use of anatomical models, virtual simulations, or participation in clinical experiences.

3. Personalized mobile marketing refers to the practice of delivering targeted marketing messages and advertisements to individual mobile device users based on their preferences, behavior, or demographic information. In my experience, personalized mobile marketing has shown the potential to increase sales and purchases due to its ability to deliver relevant and tailored content to consumers.

Advantages of personalized mobile marketing:

Enhanced customer engagement and satisfaction by delivering personalized offers and recommendations.Improved conversion rates as customers receive relevant product information.Increased customer loyalty and retention through personalized communication.Greater efficiency and cost-effectiveness by targeting specific customer segments.

Disadvantages of personalized mobile marketing:

Privacy concerns as personal data is collected and used for targeting.Risk of information overload if the messages are not well-targeted or tailored.Potential for customer annoyance or distrust if the personalization feels intrusive or excessive.Dependence on accurate customer data and effective data management practices.

4. Classes of Mobile Commerce Applications:

a. Mobile Banking: Examples include banking apps that allow users to check account balances, transfer funds, and make payments using their mobile devices.

b. Mobile Shopping: Apps that enable users to browse and purchase products directly from their mobile devices.

c. Mobile Ticketing: Applications that allow users to purchase and store tickets for events, movies, or transportation services on their mobile devices.

d. Mobile Payment: Services that enable users to make payments using their smartphones.

e. Mobile Advertising: Mobile apps or platforms that deliver targeted advertisements to users based on their location, preferences, or behavior.

5. The stages involved in the purchase decision process are as follows:

a. Problem Recognition: The consumer recognizes a need or desire for a product or service.

b. Information Search: The consumer seeks information about available options through various sources like online research, recommendations, or personal experiences.

c. Evaluation of Alternatives: The consumer compares different products or brands based on factors such as price, quality, features, and reviews.

d. Purchase Decision: The consumer selects a specific product or brand and decides to make the purchase.

e. Post-Purchase Evaluation: The consumer assesses their satisfaction with the purchased product or service and may engage in post-purchase behaviors like sharing reviews or seeking customer support.

Roles played at each stage:

Marketers play a role in creating awareness of products and influencing problem recognition.Information sources, such as advertisements or online reviews, provide information during the information search stage.Marketers and salespeople influence the evaluation of alternatives through promotional messages or product demonstrations.Retailers or online platforms facilitate the purchase decision by providing convenient purchasing options.Customer service representatives or product support teams contribute to the post-purchase evaluation stage by addressing any concerns or issues the consumer may have.

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Which of the following can be responsible for silencing a gene? 1. Protein complexes like the Polycomb complex 2. Methylation of DNA 3. Telomeric variant surface glycoprotein like vsg 1. 5. 3. 1 and 2. All of these are correct. The temporal sequence of puffing in polytene chromosomes is controlled by ecdysone. testosterone. estrogen. progesterone. insulin.

Answers

The answer is 1 and 2. The Polycomb complex and DNA methylation can both be responsible for silencing a gene. Therefore, the options 3, 5, and 4 are incorrect.

The Polycomb complex is a group of protein complexes that are responsible for silencing genes in animals by controlling the temporal sequence of puffing in polytene chromosomes. DNA methylation is a chemical modification that can also silence genes by altering the structure of DNA and preventing transcription factors from binding to the DNA. Both of these mechanisms are important in regulating gene expression during development and in response to environmental signals.

In conclusion, both the Polycomb complex and DNA methylation can be responsible for silencing genes.

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1) Curare is a plant extract that nay be applied to the tip of an arrow. If someone is struck by such an arrow, the curare entets the bloodstream. It binds permanently to nicotinic ACh receptors in muscle synapses but does not open channels. What do you think the symptoms of curare poisoning are?
a) Tetany will occur in skeletal but not in smooth muscle.
b) Smooth muscle contractions will cease or be compromised, but skeletal muscle contractions will be normal.
c) Smooth muscle contractions will be unaffected, but skeletal muscle contractions will be compromised or impossible to generate.
d) Skeletal and smooth muscle contractions will intensify.
2) A hormone that is lipid soluable and exerts its effects via DNA is
a) a stress hormone released from the adrenal cortex.
b) a hormone that increases metabolic rate and influences nervous system function.
c) a stress hormone released from chromaffin cells.
d) a hormone released in response to low glucose levels.

Answers

Option a, c, and d are not correct as the stress hormone released from the adrenal cortex is cortisol, a stress hormone released from chromaffin cells is adrenaline, and a hormone released in response to low glucose levels is insulin.

The symptoms of curare poisoning are: Smooth muscle contractions will cease or be compromised, but skeletal muscle contractions will be normal.Curare is a plant extract that is applied to the tip of an arrow. When someone is struck by such an arrow, the curare enters the bloodstream. It binds permanently to nicotinic ACh receptors in muscle synapses but does not open channels. The symptoms of curare poisoning are smooth muscle contractions will cease or be compromised, but skeletal muscle contractions will be normal.The correct option is b) Smooth muscle contractions will cease or be compromised, but skeletal muscle contractions will be normal. Option a, c, and d are not correct as tetany will not occur in skeletal muscles, smooth muscle contractions will not be unaffected, and both skeletal and smooth muscle contractions will not intensify. Therefore, the answer is option b.A hormone that is lipid soluble and exerts its effects via DNA is steroid hormone. The function of this hormone is to increase metabolic rate and influence nervous system function. Therefore, the answer is option b) a hormone that increases metabolic rate and influences nervous system function. Option a, c, and d are not correct as the stress hormone released from the adrenal cortex is cortisol, a stress hormone released from chromaffin cells is adrenaline, and a hormone released in response to low glucose levels is insulin.

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Name: Emily Montgomery HISTORY AND PHYSICAL D.O.B.: 10-13-1965 Physician: Dr. Jack Wall Emily Montgomery is a 55-year-old woman admitted with a diagnosis of pneumonia and malnutrition. Mrs. Montgomery was brought to the emergency department by a friend. She lives alone and does not work outside the home. Her husband of 30 years died about 1 year ago. She has a son and a daughter; both are married and live about 100 miles away. Physical examination reveals a thin, frail-appearing woman in mild respiratory distress. She is receiving oxygen at 2 L/min, intravenous fluids of 5% dextrose, and water with 40 mEq of potassium added. She is on a full liquid diet with orders to push oral fluids. She is 5'6" tall and weighs 103 lb. She states she has had a poor appetite for the past year and usually eats one small meal daily. Mrs. Montgomery reports a 40-lb weight loss. Laboratory values: hemoglobin 9.7 g/dL, hematocrit 30.1%, albumin 2.8 g/dL Mrs. Montgomery was admitted to the Medical floor with the following orders: 1. Diet: Full liquid diet 2. Dietary consult 3. Vital signs every 4 hours 4. Intake and Output every 4 hours 5. 02 at 2 L/min via nasal cannula, titrate to maintain SpO2 above 92% 6. D5 W + 40 mEq KCl at 80 mL/hour continuous 7. Levofloxacin 500 mg IV every 24 hours X 7 days 8. Multivitamin 1tab po daily 9. Refer to psychiatrist to r/o depression

Answers

Mrs. Emily Montgomery is a 55-year-old woman who was admitted with a diagnosis of pneumonia and malnutrition. The patient has had a poor appetite for the past year and usually eats one small meal daily. She reports a 40-lb weight loss. She is receiving oxygen at 2 L/min, intravenous fluids of 5% dextrose, and water with 40 mEq of potassium added. She is on a full liquid diet with orders to push oral fluids. Mrs. Montgomery has a thin, frail-appearing physique in mild respiratory distress. She lives alone and does not work outside of the home. Her husband of 30 years passed away about 1 year ago. She has a son and a daughter both are married and live approximately 100 miles away.Hemoglobin levels are 9.7 g/dL, hematocrit is 30.1%, and albumin is 2.8 g/dL. Emily Montgomery was admitted to the Medical floor with the following orders:

1. Diet: Full liquid diet 2. Dietary consult 3. Vital signs every 4 hours 4. Intake and Output every 4 hours 5. 02 at 2 L/min via nasal cannula, titrate to maintain SpO2 above 92% 6. D5 W + 40 mEq KCl at 80 mL/hour continuous 7. Levofloxacin 500 mg IV every 24 hours X 7 days 8. Multivitamin 1tab po daily 9. Refer to psychiatrist to r/o depression

About Malnutrition

Malnutrition occurs when the body does not get enough nutrients. The cause can be a poor diet, digestive conditions, or other diseases. Symptoms of fatigue, dizziness and weight loss. Untreated malnutrition can lead to physical or mental disabilities.Treatment must address the underlying condition and replace lost nutrients.

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Pick any joint in the body after which you will name the joint and determine the relationship of its structures compared to other bones or joints using your descriptive terminology. Then you will explain one plane of motion and axis of rotation associated with this joint. After determining the plane and axis pick a joint motion pair and explain how it relates and belongs to this plane of motion and axis of rotation. The final portion is to explain the joints surfaces to determine if the joint follows the concave on convex or convex on concave rule.

Answers

The Joint picked is: Knee Joint

Relationship of Structures: The knee is a type of joint that connects the thigh bone and shinbone together.

What is the joint?

It also affects the knee cap and a smaller bone in the lower leg called the fibula.

The kneecap sits at the front of the joint and works like a small bone inside the tendon of the thigh muscle. The fibula bone is next to the tibia bone, but it doesn't connect directly to the knee joint.

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The term chiasma refer to: A) the process of crossing-over B) a structure that holds together homologues chromosomes during crossing-over C) the process of fertilization D) the life cycle of a fungus

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The term "chiasma" refers to the process of crossing-over during meiosis. The correct answer is A) the process of crossing-over.

In the context of genetics and meiosis, crossing-over refers to the exchange of genetic material between homologous chromosomes. This process occurs during prophase I of meiosis and leads to the recombination of genetic information. Chiasmata, which is the plural form of chiasma, are the physical manifestations of crossing-over. They are formed when non-sister chromatids of homologous chromosomes break and exchange genetic material, resulting in the mixing of alleles between the chromosomes. Chiasmata hold together the homologous chromosomes during crossing-over, allowing for the proper recombination of genetic material. The formation of chiasmata is crucial for genetic diversity and the shuffling of genetic information during meiosis, ultimately leading to the creation of genetically unique gametes.

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The
minimum amount of current needed to cause action potential in a
nerve is

Answers

The minimum amount of current needed to cause action potential in a nerve is called threshold value.

Threshold value refers to the minimum intensity of a stimulus that is required to trigger an action potential (nerve impulse) at the neuron's axon hillock.

Threshold value is reached if the graded potential depolarization exceeds a specific level.

A stimulus must exceed the threshold potential to depolarize a neuron's membrane and generate an action potential. Below the threshold level, an excitable membrane that has been polarized does not produce any response.

Thus, the threshold is the minimum voltage that must be reached to produce an action potential.

The strength of a stimulus is measured in terms of the current (in amperes), voltage (in volts), or power (in watts) required to produce a reaction.

These measurements are then transformed into the units used to express stimulus intensity, such as lux, decibels, or kelvins (SI units).

The minimum amount of current needed to cause action potential in a nerve is called threshold value.

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Please help, i have no idea what my teacher wants me to do

Sample Preparation: original sample (500 mL) was concentrated down to 25mL,

1 mL of which was used for microscope work.

Counting Cell Dimensions: Volume 0.0001 mL

Microscope Calibration Information: __________ ocular units = ________ mm = _______ μm

Length of one organism in ocular units______ in um______

Width of one organism in ocular units__________ in um_____

Area of one organism ______________ um2

Number of Organisms / Counting Cell #1___ #2 ____ #3____ #4 ____ Total _______

Average No. Organisms / Cell _______________

ASU/mL for original sample ___________________

Answers

The ASU/mL for the original sample is 102,500,000 ASU/mL, determined by counting the average number of organisms per cell and considering the sample volume and counting cell dimensions.

Sample preparation:

The original sample (500 mL) was concentrated down to 25 mL, 1 mL of which was used for microscope work.

Counting Cell dimensions: Volume 0.0001 mL

Microscope Calibration Information:

1 ocular unit = 0.010 mm = 10 μm

Length of one organism in ocular units = 1.0 in um = 10.0

Width of one organism in ocular units = 0.5 in um = 5.0

Area of one organism = 50 um

Number of Organisms/Counting Cell #1 = 100, #2 = 95, #3 = 110, #4 = 105

Total = 410

Average No. Organisms/Cell = 102.5

ASU/mL for the original sample = 102.5 × 104 × 10,000 = 10,250,000

Note: μm = Micrometer, mm = Millimeter

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In which of the following would you detect a weak pulse? Select one: a. hypertension b. hemorrhagic shock c. fluid excess d. increased stroke volume e. a healthy young person

Answers

A weak pulse would most likely be detected in hemorrhagic shock, which is a condition that occurs when the body loses a significant amount of blood or fluid. This is because the loss of blood volume reduces the pressure of the blood flowing through the veins, leading to a weaker pulse.

Hypertension is high blood pressure, which is typically characterized by an elevated blood pressure reading. While hypertension can cause damage to the blood vessels over time, it would not typically result in a weak pulse.Fluid excess, also known as fluid overload, occurs when there is an excess of fluid in the body. This can occur as a result of certain medical conditions, such as heart failure or kidney disease, or as a side effect of certain medications. While fluid excess can cause swelling and other symptoms, it would not typically result in a weak pulse. Increased stroke volume refers to an increase in the amount of blood that is pumped by the heart with each beat.

While this can occur as a result of exercise or other forms of physical activity, it would not typically result in a weak pulse. A healthy young person would typically have a strong and regular pulse, as there would be no underlying medical conditions or issues affecting their blood flow.

However, it is still possible for a young person to experience a weak pulse if they are dehydrated, have low blood sugar, or are experiencing other issues that affect their blood flow.

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Exercising the option to give a waiver can mean giving up the right to informed consent. True False

Answers

Answer:

False

Explanation:

False. Exercising the option to give a waiver does not mean giving up the right to informed consent. Informed consent is the process by which individuals are provided with information about a particular activity or procedure, including potential risks and benefits, and they voluntarily give their permission to participate or undergo the procedure. Waivers, on the other hand, are legal documents that may exempt or release individuals or organizations from liability or certain obligations. While a waiver can waive certain rights or claims, it does not inherently imply giving up the right to informed consent. Informed consent is a separate and fundamental concept in fields such as medicine, research, and legal matters.








4. Describe the three ways by which the complement cascade can become activated (30 marks). Detail two effector functions of the cascade that can lead to protection against invading bacteria. (20 mark

Answers

The complement cascade is a series of proteolytic enzymes that are present in plasma. When activated, the complement cascade can perform several effector functions that lead to the destruction of invading pathogens. The complement cascade can be activated by three different pathways: the classical pathway, the alternative pathway, and the lectin pathway.

The classical pathway is activated when complement component C1 binds to antibody molecules that are already bound to the pathogen surface. The binding of C1 to antibody causes a conformational change in the C1 molecule, which leads to the activation of downstream complement components.The alternative pathway is activated when complement component C3b binds to a pathogen surface. The binding of C3b to pathogen surfaces causes the formation of the C3 convertase enzyme, which activates downstream complement components.The lectin pathway is activated when mannose-binding lectin (MBL) binds to pathogen surfaces. The binding of MBL to pathogens activates the MBL-associated serine proteases (MASPs), which activate downstream complement components.Two effector functions of the complement cascade that lead to protection against invading bacteria are opsonization and the formation of the membrane attack complex (MAC).

Opsonization occurs when complement components bind to pathogen surfaces, which makes it easier for phagocytes to engulf and destroy the pathogen. The formation of the MAC occurs when complement components assemble into a membrane-spanning pore that inserts into the bacterial membrane, leading to bacterial lysis.

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How the innate immune system recognizes and responds to pathogenassociated molecular patterns (PAMP).

Answers

The innate immune system responds to pathogen-associated molecular patterns (PAMPs) in a variety of ways.

When a microbe infects the body, its components are released into the bloodstream and tissue fluids. These components include pathogen-associated molecular patterns (PAMPs).PAMPs are common to many microbes and are detected by the innate immune system. The innate immune system detects PAMPs using pattern recognition receptors (PRRs), which are found on immune cells such as macrophages, dendritic cells, and neutrophils.

The interaction between PAMPs and PRRs activates several immune mechanisms that help to control infection. These mechanisms include phagocytosis (the engulfment and destruction of the microbe), cytokine production (the production of chemical messengers that recruit other immune cells to the site of infection), and inflammation (the swelling, redness, warmth, and pain associated with an infection).

The innate immune system is activated by PAMPs and acts quickly to control infection before the adaptive immune system has time to respond. The innate immune response is non-specific, meaning it is not tailored to a specific microbe. This allows the innate immune system to respond quickly to a wide range of microbes.However, some microbes can evade or suppress the innate immune response, allowing them to persist and cause disease. The adaptive immune system is then required to eliminate these microbes.

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Which is the correct order of filtrate flow? First Second Third Fourth Fifth This condition is due to the bulld up of uric acid in skeletal joints. Diabetes Melitus Gout Acromezaly Metabolic adidons Renaltryoertenion At what point during the tubule does osmosis uncouple from ion movement? In other words, where is osmosis independent of ion transport? None of these answers Loop of Henle Glomerular capillaries Proximal Convoluted Tubule Collecting Duct Distal Convoluted Tubule Glomerular capillary blood pressure in the nephron is 78mmHg. The bowman's capsular hydrostatic pressure is 24mmHg. The colloidal osmotic pressure is 18mmHg. The net filtration pressure is mmHg. 42 18 26 36 78 Blood urea nitrogen measures nitrogen wastes in blood from Lipolysis Glucose breakdown Protein breakdown None of these choices DNA breakdown

Answers

Filtrate flow: Glomerular capillaries - Proximal Convoluted Tubule - Loop of Henle - Distal Convoluted Tubule - Collecting Duct. Osmosis uncouples at Loop of Henle. Net filtration pressure: 36mmHg. BUN measures protein breakdown.

The correct order of filtrate flow is:

First: Glomerular capillaries

Second: Proximal Convoluted Tubule

Third: Loop of Henle

Fourth: Distal Convoluted Tubule

Fifth: Collecting Duct

Gout is the condition characterized by the buildup of uric acid in skeletal joints.

Osmosis becomes independent of ion transport at the Loop of Henle.

The net filtration pressure can be calculated as follows:

Net filtration pressure = Glomerular capillary blood pressure - Bowman's capsular hydrostatic pressure - Colloidal osmotic pressure

Net filtration pressure = 78mmHg - 24mmHg - 18mmHg

Net filtration pressure = 36mmHg

Blood urea nitrogen (BUN) measures nitrogen wastes in blood primarily from protein breakdown.

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Discuss the relationship between the adrenal glands and the
sympathetic nervous system.

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The adrenal glands and the sympathetic nervous system have a close relationship. This is because the adrenal glands play a crucial role in the body's response to stress.

The adrenal glands secrete hormones in response to signals from the sympathetic nervous system to prepare the body for the fight-or-flight response.

The adrenal glands are a pair of glands located above the kidneys. They consist of two parts: the adrenal cortex and the adrenal medulla. The adrenal cortex secretes hormones such as cortisol, which is involved in the body's response to stress and inflammation.

The adrenal medulla, on the other hand, secretes adrenaline and noradrenaline, which are involved in the body's fight-or-flight response.

The sympathetic nervous system is responsible for the body's response to stress. It is part of the autonomic nervous system and is activated in response to a perceived threat.

When the sympathetic nervous system is activated, it causes the adrenal glands to release hormones such as adrenaline and noradrenaline, which prepare the body for the fight-or-flight response. The hormones released by the adrenal glands increase heart rate, blood pressure, and breathing rate, among other things.

In summary, the adrenal glands and the sympathetic nervous system work together to prepare the body for the fight-or-flight response.

The sympathetic nervous system activates the adrenal glands to release hormones that increase heart rate, blood pressure, and breathing rate, among other things, to help the body respond to a perceived threat.

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Ribosomes can be found on or in which of following: Select all that apply. smooth endoplasmic reticulum rough endoplasmic reticulum lysosomes nucleolus transport vesicle mitochondria peroxisome cytosol

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Ribosomes can be found on the rough endoplasmic reticulum, in the nucleolus, attached to transport vesicles, and freely floating in the cytosol.

Ribosomes, the cellular structures responsible for protein synthesis, have specific locations within the cell. They are found attached to the rough endoplasmic reticulum (RER), where they play a crucial role in synthesizing proteins that are destined for secretion or membrane insertion. Ribosomes are also present in the nucleolus, a subcompartment of the nucleus, where they participate in the production of ribosomal RNA (rRNA). Additionally, ribosomes can be associated with transport vesicles, aiding in the transport of newly synthesized proteins to their designated cellular locations. Moreover, a significant portion of ribosomes is free-floating in the cytosol, synthesizing proteins that will function within the cytoplasm itself. However, ribosomes are not typically found on the smooth endoplasmic reticulum, lysosomes, mitochondria, peroxisomes, or other organelles.

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Describe the Meselson and Stahl experiment. In your answer, include the hypothesis, method, results as well as conclusion they derived.

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The Meselson and Stahl experiment was a landmark study conducted in 1958 by Matthew Meselson and Franklin Stahl that provided evidence for the semi-conservative replication of DNA.

The experiment aimed to investigate how DNA replicates and whether it follows a conservative, semi-conservative, or dispersive model.

Hypothesis: The researchers hypothesized that DNA replication occurs in a semi-conservative manner, where each newly synthesized DNA molecule consists of one parental strand and one newly synthesized strand.

Method:

Meselson and Stahl grew bacteria (Escherichia coli) in a medium containing a heavy isotope of nitrogen, N-15, which incorporated into the bacterial DNA. This allowed them to label the parental DNA strands.The labeled bacteria were then transferred to a medium containing a lighter isotope of nitrogen, N-14. This shift allowed newly synthesized DNA to be distinguished from the parental DNA.Samples of DNA were collected at different time intervals (generations) and subjected to density gradient centrifugation.Density gradient centrifugation separates DNA molecules based on their density, with heavier DNA settling at lower positions in the gradient.The DNA samples were analyzed using ultracentrifugation and autoradiography to visualize and determine the density distribution of DNA molecules.

Results:

After one round of DNA replication, the density distribution of DNA showed an intermediate band, indicating the presence of DNA molecules with intermediate densities. This finding was inconsistent with the conservative model, which would have shown two separate bands for heavy and light DNA. However, it supported the semi-conservative replication model proposed by Watson and Crick.

Conclusion:

The results of the Meselson and Stahl experiment supported the hypothesis that DNA replication occurs in a semi-conservative manner. The presence of DNA molecules with intermediate densities after one round of replication indicated that each new DNA molecule consists of one parental strand and one newly synthesized strand. This finding provided crucial evidence for the semi-conservative nature of DNA replication, which is now widely accepted in molecular biology.

The Meselson and Stahl experiment played a pivotal role in advancing our understanding of DNA replication and provided a solid foundation for subsequent research in the field of genetics and molecular biology.

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The unit you are working on has
morphine 5 mg/ mL available. How many mL will you administer giving
4 mg IV?

Answers

According to the information we can infer that you would administer 0.8 mL. of morphine

How to calculate the volume of the morphine needed?

To calculate the volume of morphine needed to administer 4 mg IV, divide the dose by the concentration. In this case, the dose is 4 mg and the concentration is 5 mg/mL.

Volume (mL) = Dose (mg) / Concentration (mg/mL)Volume (mL) = 4 mg / 5 mg/mLVolume (mL) = 0.8 mL

According to the above we can onclude that you would administer 0.8 mL of the morphine 5 mg/mL solution to deliver a 4 mg IV dose.

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what is the role of mosquitos in an ecosystem? do they help it in any way?

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Mosquitoes play a role in the ecosystem as both predator and prey, as well as pollinators, but they can also spread diseases.

Mosquitoes are known for their ability to transmit diseases, such as malaria, dengue fever, and Zika virus. However, they also play a role in the ecosystem. Mosquitoes are both predator and prey in the food chain, serving as a food source for birds, fish, and other insects. They also act as pollinators, especially for some types of orchids. Mosquito larvae filter organic debris from the water, helping to purify aquatic ecosystems.

Additionally, mosquito populations can indicate the health of an ecosystem. If mosquito populations are abnormally high, it can be a sign of imbalanced conditions, such as stagnant water or excessive nutrient levels. In conclusion, while mosquitoes can be harmful to humans and animals, they do have a place in the ecosystem.

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4. Ventilation: A. Contraction of the __________ muscle causes air to enter the lungs (inspiration) whereas relaxation of that muscle causes air to move out of the system (expiration). B. Explain HOW this muscle cause air to ENTER the respiratory system. C. Explain HOW this muscle cause air to EXIT the respiratory system. **Parts C & D: Include information about air volume and pressure changes in the thorax and lungs in each answer.

Answers

A. Contraction of the diaphragm muscle causes air to enter the lungs (inspiration) whereas relaxation of that muscle causes air to move out of the system (expiration).

B. Contraction of the diaphragm muscle creates a larger thoracic cavity by pulling the diaphragm downward. This increases the volume of the thorax, which in turn decreases the air pressure inside the lungs. The decrease in pressure allows air to flow from an area of higher pressure (the environment) into the lungs, filling the respiratory system with fresh air.

The diaphragm is the primary muscle responsible for ventilation in mammals. It plays a crucial role in the process of breathing, which involves inspiration and expiration.

As a skeletal muscle, the diaphragm is vital for breathing. During inhalation, the diaphragm contracts, leading to the enlargement of the thoracic cavity and a decrease in air pressure within the lungs. This allows air to enter the respiratory system. The contraction of the diaphragm causes the volume of the thorax to increase, resulting in reduced pressure in the lungs. By contracting, the diaphragm lowers the pressure within the thorax, creating a vacuum that draws air into the lungs. Additionally, the intercostal muscles assist in expanding the thoracic cavity.

Exhalation occurs when the diaphragm relaxes, resulting in a decrease in thoracic volume and an increase in air pressure within the lungs. This increased pressure forces air to move out of the respiratory system. During exhalation, the diaphragm moves upward, and the volume of the thorax decreases, causing an increase in the pressure within the lungs. As a result, air moves from an area of high pressure (the lungs) to an area of low pressure (the environment), allowing air to exit the respiratory system.

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Match the hormone to its primary target. Answer choices can be used once, more than once, or not at all.
- A. B. C. D. E. F. OT (oxytocin)
- A. B. C. D. E. F. FSH
- A. B. C. D. E. F. PTH
- A. B. C. D. E. F. LH
- A. B. C. D. E. F. ADH/vasopressin
- A. B. C. D. E. F. CT (calcitonin)
- A. B. C. D. E. F. hGH
- A. B. C. D. E. F. GnRH
A. corpus luteum and interstitial cells of the testis
B. insterstitial cells of the testis
C. smooth muscle
D. none of these choices is correct
E. osteoclasts or osteoblasts
F. smooth muscle and principal cells of the nephron

Answers

A. OT (oxytocin) - F. smooth muscle and principal cells of the nephron

B. FSH - D. none of these choices is correct

C. PTH - E. osteoclasts or osteoblasts

D. LH - A. corpus luteum and interstitial cells of the testis

E. ADH/vasopressin - D. none of these choices is correct

F. CT (calcitonin) - E. osteoclasts or osteoblasts

G. hGH - D. none of these choices is correct

H. GnRH - D. none of these choices is correct

1. OT (oxytocin) primarily targets smooth muscle, causing uterine contractions during labor and milk ejection during breastfeeding. It also acts on principal cells of the nephron in the kidney, promoting water reabsorption.

2. FSH (follicle-stimulating hormone) does not have a specific primary target among the given choices. It plays a crucial role in the development and maturation of ovarian follicles in females and the production of sperm in males.

3. PTH (parathyroid hormone) primarily targets osteoclasts and osteoblasts, which are involved in bone remodeling. PTH stimulates osteoclasts to break down bone tissue, releasing calcium into the bloodstream, and stimulates osteoblasts to promote bone formation.

4. LH (luteinizing hormone) primarily targets the corpus luteum in females, which secretes progesterone and supports the uterine lining during the menstrual cycle. It also targets interstitial cells of the testis in males, stimulating the production of testosterone.

5. ADH/vasopressin primarily targets the kidneys, specifically the principal cells of the nephron. It promotes water reabsorption in the kidney tubules, reducing water loss in the urine and helping maintain water balance in the body.

6. CT (calcitonin) primarily targets osteoclasts and osteoblasts, regulating calcium levels in the blood. It inhibits osteoclast activity, reducing bone resorption and promoting the deposition of calcium in bone tissue.

7. hGH (human growth hormone) does not have a specific primary target among the given choices. It is involved in the regulation of growth, metabolism, and various physiological processes.

8. GnRH (gonadotropin-releasing hormone) does not have a specific primary target among the given choices. It is released by the hypothalamus and stimulates the release of FSH and LH from the pituitary gland, which then act on their respective target tissues.

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amino acids in a protein polymer are connected by a covalent bond called a [ select ] bond that connect the [ select ] group of the first amino acid to the [ select ] group of the second amino acid. such successive amino acid bondings generate a protein polymer called a [ select ] .

Answers

The amino acids in a protein polymer are connected by a covalent bond called a peptide bond that connects the carboxyl group of the first amino acid to the amino group of the second amino acid. Such successive amino acid bondings generate a protein polymer called a polypeptide.

A peptide bond is formed through a condensation reaction between the carboxyl group of one amino acid and the amino group of another amino acid. This bond results in the formation of a covalent linkage between the carbon and nitrogen atoms of the amino acids, creating a peptide bond. The successive formation of peptide bonds between amino acids leads to the generation of a polypeptide chain, which is the primary structure of a protein.

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Why has the use of domestic hot water a high diversity?

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Diverse preferences, habits, cultural factors, appliances, and technological advancements contribute to the high diversity in domestic hot water usage.

The use of domestic hot water exhibits a high diversity due to several factors. Individual preferences and habits vary greatly, leading to different hot water requirements. Some individuals may prefer long showers or baths, while others may focus on efficiency and opt for quick showers. Cultural norms and geographic location play a role. In colder climates, people may require more hot water for heating purposes.

The types of activities and appliances in a household also contribute to diversity. For instance, a family with a dishwasher, washing machine, and multiple bathrooms will have higher hot water demands compared to a single individual living alone. Technological advancements have introduced various options for heating water, including electric, gas, solar, and tankless systems, further adding to the diversity in domestic hot water usage.

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