Peptides can be separated using an ion‑exchange column based on their isoelectric (pI) values. At which pH values would two different peptides, one with a pI of 5.55.5 and the other with a pI of 9.29.2 , bind to a cation‑ and anion‑exchange column? Each peptide may be capable of binding to each column at more than one pH value.

Answers

Answer 1

Answer:

At pH of 3.6, Peptide A and B will bind to the anion-exchange column, while neither peptide will bind to the cation-exchange column.

At pH of 6.6, Peptide A will bind to the cation-exchange column while Peptide B will bind to the anion-exchange column.

At pH of 10.0, Peptide A and B will bind to the cation-exchange column, whereas neither peptide will bind to the anion-exchange column.

Note: The question is incomplete. A sample complete question is found in the attachment below.

Explanation:

If pH is greater than pI, the net charge on the peptide is negative.

When pH = pI, the net charge is zero

When pH is less than pI, the net charge on the peptide is positive.

A positively charged peptide will bind to an anion-exchange column, whereas a negatively charged peptide will bind to a cation-exchange column.

At pH of 3.6, Peptide A and B will bind to the anion-exchange column, while neither peptide will bind to the cation-exchange column.

At pH of 6.6, Peptide A will bind to the cation-exchange column while Peptide B will bind to the anion-exchange column.

At pH of 10.0, Peptide A and B will bind to the cation-exchange column, whereas neither peptide will bind to the anion-exchange column.

Peptides Can Be Separated Using An Ionexchange Column Based On Their Isoelectric (pI) Values. At Which

Related Questions

How many valence electrons will atoms in group 16 of the periodic table have?

Answers

Answer:

6 valence electrons

Explanation:

Which particles can be found in the nucleus of an atom?
-protons and neutrons
-protons and electrons
-alpha particles
-neutrons and electrons

Answers

Answer:

proteins and neutron.

Answer:

Explanation:

i have a few question please help its due in a few hours

1. Determine the specific heat of a certain metal if a 450 gram sample of it loses 34,500J of heat as its temperature drops by 97K

2. The temperature of a 25 gram sample of a certain metal drops by 103 K as it loses 2600 joules of heat. What is the specific heat of the metal?

3.What is the final temperature after 840 Joules is absorbed by 10.0g of water at 298K?
(please show steps)

Answers

Answer:

1 = c = 0.79 j/g.K

2= c = 1 j/g.K

3 = T2 = 123.1 K

Explanation:

Given data:

Specific heat of metal = ?

Mass of metal = 450 g

Heat loses = 34,500 j

Temperature drop = ΔT = 97 K

Solution:

Specific heat capacity:

It is the amount of heat required to raise the temperature of one gram of substance by one degree.

Formula:

Q = m.c. ΔT

Q = amount of heat absorbed or released

m = mass of given substance

c = specific heat capacity of substance

ΔT = change in temperature

34500 j = 450 g ×c × 97 K

34500 j = 43650 g.K ×c

c = 34500 j/43650 g.K

c = 0.79 j/g.K

Given data:

Specific heat of metal = ?

Mass of metal = 25 g

Heat loses = 2600 j

Temperature drop = ΔT = 103 K

Solution:

Specific heat capacity:

It is the amount of heat required to raise the temperature of one gram of substance by one degree.

Formula:

Q = m.c. ΔT

Q = amount of heat absorbed or released

m = mass of given substance

c = specific heat capacity of substance

ΔT = change in temperature

2600 j = 25 g ×c × 103 K

2600 j = 2575 g.K ×c

c = 2600 j/2575 g.K

c = 1 j/g.K

Given data:

Mass of water = 10 g

Heat absorbed = 840 j

Initial temperature drop = 298 K

Final temperature = ?

Solution:

Formula:

Q = m.c. ΔT

Q = amount of heat absorbed or released

m = mass of given substance

c = specific heat capacity of substance

ΔT = change in temperature

ΔT = T2 - T1

840 j = 10 g ×4.18 j/g.K × [T2 - 103 K]

840 j = 41.8 J/K × [T2 - 103 K]

840 J/41.8 J/ K = T2 - 103 K

20.1 k = T2 - 103 K

T2 = 20.1 K + 103 K

T2 = 123.1 K

5. (04.03 LC)
Which of the following steps can be used to identify an acid-base reaction
Check if one element replaces another element in a compound.
Check if the ions of two compounds exchange places.
O Check whether the reactants are an acid and a base.
Check whether the products are an acid and a base.
Plz help

Answers

Answer: Check whether the reactants are an acid and a base.

Explanation:

The step to determine the acid-base reaction, it has been checked that where there has been an acid or base in the reactants or not. Thus, option C is correct.

The acid base reaction can be described as the chemical reaction involving the exchange of hydrogen ions in between the reactant species.

The acid base reaction has been mediated in the system where there has been the presence of an acid or base as the reactant in order to accept or donate hydrogen ions.

Thus, to determine the acid-base reaction, it has been checked that where there has been an acid or base in the reactants or not. Thus, option C is correct.

For more information about the acid-base reaction, refer to the link:

https://brainly.com/question/25280500

The highest mountain in North America is Mt. McKinley at 3.848 miles high. How many kilometers is that?

Answers

6.192755712 km is the answer

Help please
C2H4O2

1. How many different atoms are in the compound above?________

2. How many atoms of hydrogen?__________

3. How many total atoms are in this compound?________

4. If a big 2 were added in front of the formula, what would that mean?

Answers

Answer:

1. There are three different atoms

2. There are 4 hydrogen

3. There are 8 atoms in the compound

4. All the atoms are double the number

Ions bonds are strong?
True or False

Answers

Answer:

Ture

Explanation:

Answer:

true

Explanation:

ions bond are those bonds which are made with the exchange of electrons between metals and nonmetals.Thus they are very strong.

Write 0.0000745 in scientific notation.

Answers

Answer:

the answer is 7.45 x 10^-5

Move the decimal point 5 places to the right.

In your own words describe a chemical change and physical change.

Answers

Physical change is a change where physical property of a substance change. Like shape, size etc...
It is temporary and reversible I.e the change can be undone by simple physical methods.
It does not involves any energy.
No new substance is formed.

While the chemical change is a change in which the chemical properties of a substance change. Like smell etc..including it’s composition.

Also it is permanent and irreversible.
It involves absorption and production of energy.
New substance is formed.
Hope it helped you.

Which compound forms a powerful acid and also contains a halogen?
A. H2S
B.HBr
C.Li2O
D.LiBr

Answers

Answer:

HBr

Explanation:

Answer:

The answer is in fact HBr

Explanation:

I took the quiz

In any measurement, which digit is the uncertain digit?

Answers

Answer:

The last digit is estimated

Explanation:

the last digit of any measurement is estimated, and it is this estimated digit that reflects uncertainty. The more decimal places there are the less uncertainty there is.

The number of significant figures is uncertain in a number that ends with a zero to the left of the decimal point location. The zeros in the measurement 1,300 grams could be significant or they could simply indicate where the decimal point is located.

According to the concept of significant figures, the last digit is uncertain digit in a measurement.

What are significant figures?

Significant figures are used for establishment of a number which is presented in the form of digits. These digits give a meaningful representation to the numbers.

The significant figures are the significant digits which convey the meaning according to the accuracy. These provide precision to the numbers and hence are called as significant numbers.There are rules for counting significant figures which are as follows:

1)All non-zero digits are significant .

2)All zeroes which occur between non-zero digits are significant.

3)All zeroes to the left and right of a non-zero digit are not significant.

4) All zeroes on right of decimal are significant if a non-zero number follows them.

5)All zeroes on right side of non-zero digit are significant.

Answer:

Neon

Explanation:

hskamamsahaksnavshaiaaa

irieiwkaaj

Determine the number of atoms in the following chemical formula: Ba(OH)2

Answers

Answer:

Explanation:

Ba,1 O,2 H,2

the somes are 5

The density of an object is .835 g/cm3. If its volume is 34 cm3, what is the mass of the object? (Density: D = m/v)

Answers

Answer:

The answer is 28.39 g

Explanation:

The mass of a substance when given the density and volume can be found by using the formula

mass = Density × volume

From the question we have

mass = 0.835 × 34

We have the final answer as

28.39 g

Answers

Answer

2.626984127 m

Explanation:

You have to know the equation that relates wavelength, frequency, and velocity (it's like speed but a bit different).

v = f x λ

where:

v = velocity

f = frequency

λ = Wavelength

Rearrange to make λ subject:

λ = v / f

We've been given 331 as the speed, 126 as the frequency. Sub it into the equation:

331 / 126 = 2.626984127 m

PLEASE ASAP
A calcium atom has 20 protons, 18 electrons, and 20 neutrons. What is the charge on the atom?

Answers

Answer:

Ca2+ represents an ion with 20 protons and 18 electrons.

Explanation:

A calcium atom has 20 protons and 20 electrons. The 2+ charge next to the symbol indicates a loss of two electrons: 20-2=18.

what is the metal that is grouped with metalloids

Answers

Answer:

The post-transition metals cluster to the lower left of this line. Metalloids: The metalloids are boron (B), silicon (Si), germanium (Ge), arsenic (As), antimony (Sb), tellurium (Te) and polonium (Po). They form the staircase that represents the gradual transition from metals to nonmetals.

Explanation: i googled it

The metal that is grouped with metalloids are

boron, silicon, germanium,arsenic, antimony, and tellurium.

A metalloid is a type of element which possesses the characteristics of both the metals and the non-metals.

Metals are those elements that are good conductors of heat and electricity while non-metals are those elements that neither conducts heat nor electricity.

The characteristics of the metalloids listed above are:

They conduct heat only at higher temperatures.

The metalloids that can conduct electricity are called semi-conductors.

They are shiny like metals but brittle like non-metals.

Therefore, since these elements share both the properties of metals and non-metals, they are called the metalloids.

Learn more here:

https://brainly.com/question/6499600

how many molecules are contained in 0.254 moles of diarsenic trioxide

Answers

Answer:

1.53 × 10²³ molecules As₂O₃

Explanation:

Step 1: Define

Diarsenic Trioxide - As₂O₃

Avagadro's number: 6.02 × 10²³ atoms, molecules, formula units, etc.

Step 2: Use Dimensional Analysis

[tex]0.254 \hspace{3} mol \hspace{3} As_2O_3(\frac{6.02(10)^{23} \hspace{3} molecules \hspace{3} As_2O_3}{1 \hspace{3} mol \hspace{3} As_2O_3} )[/tex] = 1.52908 × 10²³ molecules As₂O₃

Step 3: Simplify

We have 3 sig figs.

1.52908 × 10²³ molecules As₂O₃ ≈ 1.53 × 10²³ molecules As₂O₃

Who do you think should be responsible for cleaning up all the space junk orbiting the earth?
Please I really really needed help

Answers

Answer:

astronots there already up there so they might as well do it.

Neil is comparing the shifts in light patterns of two distant galaxies. Using a powerful telescope, he records the light shifts of each galaxy every 20 seconds (s) for six hours (hr). After sharing his data with Betty, another astronomer, she says that this is not an experiment.

Which of the following statements supports Betty's claim?

A.The hypothesis is actually the first step in the scientific method.
B.The hypothesis guides the researcher towards the results of the experiment.
C.The hypothesis provides the steps needed to analyze the data to reach conclusions.
D.The hypothesis provides a prediction about what might happen during the experiment.

Answers

Answer:c

Explanation: can someone help me to see if this is true

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