Biology College

Provided below are recognition sequences for restriction enzymes 1 (RE1), 2 (RE2) and 3 (RE3). Arrows indicate
the positions where the enzymes digest on the two strands. Which of the following can the RE1 digested DNA ligate
to?

Answers

Answer 1

The restriction enzyme RE1-digested DNA fragment can only ligate to the RE3-digested DNA fragment since they have complementary sticky ends. The RE2-digested DNA fragment has a different sticky end sequence, so it cannot ligate to the RE1-digested DNA fragment.

Restriction enzymes are crucial tools in modern molecular biology and genetics. These enzymes recognize specific sequences of DNA and cut them at precise locations. Ligation is the process of joining two DNA fragments, often used in cloning and gene manipulation.RE1, RE2, and RE3 are restriction enzymes with different recognition sequences and sites where they cleave the DNA molecule.

Given the recognition sequences and cut sites of these enzymes, the RE1-digested DNA fragment can ligate to the RE3-digested DNA fragment, which has a complementary sticky end.The sticky end is an unpaired single-stranded region resulting from the staggered cuts made by the REs, with a few nucleotides overhanging at the cut site.

The complementary sticky end will pair with the other DNA fragment's sticky end, forming hydrogen bonds and allowing for ligation by DNA ligase. Since the RE2-digested fragment has a different recognition sequence and cut site than RE1, it cannot ligate to the RE1-digested fragment.

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Related Questions

Explain the limitation binomial nominclature (15 marks)​

Answers

Answer:

- Binomial nomenclature is a widely accepted naming system, which is used in the naming of living things. Multiple native names make it extraordinarily tough to establish an organism globally and maintain an observation of the variety of species. Thus, it creates quite a lot of confusion. To eliminate this confusion, a normal protocol came up. According to it, every organism would have one scientific title which might be utilized by everybody to establish an organism.

What is binomial nomenclature?

Binomial Nomenclature follows a universally accepted naming system, which is used to provide a scientific name to a known organism.

Binomial Nomenclature also known as the binary nomenclature.

It uses two-terms during the nomenclature of a specie (plants, animals, and living organisms) that is why it called two-term naming system.

The scientific name of an organism or binomial nomenclature consists of two words, such as the generic epithet and describes the genus and the specific epithet and refers to the species of the organism.

Both of this term is written in italic and the genus name is capitalized.

Binomial nomenclature was given by Swedish botanist and physician Carl Linnaeus. He also called the founder of modern taxonomy.

Carl Linnaeus described and classified thousands of species of plants and animals in his book Systema Naturae.In this technique, there are particular guidelines that are followed while naming organisms. This normal algorithm is relevant to plants and animals whereas giving them distinctive names inside a given system.There are two worldwide codes such as the International Code of Botanical Nomenclature (ICBN) deal with the biological nomenclature for plants and the International Code of Zoological Nomenclature (ICZN) deal with the biological nomenclature of animals.

These two codes agreed upon by all of the biologists over the world for the naming protocol. These codes ensure that every organism will get a selected identity and that identity is globally recognized.

Summary of the limitation binomial nominclature:

- Some of the main drawbacks of binomial nomenclature are: If two or more names are actually in use in compliance with the priority rule, the appropriate name will be used first and the other names will end up being synonyms because authenticity is synonymous with the senior.

. Mushroom is under............
i. Genus
ii. Donail
iii. Taxonomy
iv. Species​

Answers

Answer:

Mushrooms are classified under the categories of "Genus" and "Species" in taxonomy. The genus refers to a group or category that includes closely related species, while the species represents a specific type or kind of organism. So, the correct options for mushrooms would be "i. Genus" and "iv. Species."

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