what procedure is expected to occur in an rflp? question 6 options: genes are transferred via a dna virus. cells are treated with enzymes that remove their walls. cells are forced into a protoplast fusion. dna is digested with endonucleases.

Answers

Answer 1

In RFLP (Restriction Fragment Length Polymorphism), a procedure is expected to occur to digest the DNA with endonucleases. Endonucleases are enzymes that cut the DNA molecule at specific sites.

In this procedure, the DNA is digested with endonucleases to generate fragments that will be separated using a gel electrophoresis technique and then transferred to a nylon membrane, which will then be hybridized with probes.

These DNA fragments are then blotted onto a nylon membrane and probed with a labeled probe, which can identify specific DNA sequences. The hybridization is then detected using autoradiography. This technique is used in forensic analysis, genetic mapping, paternity testing, and disease diagnosis.

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Related Questions

consider a population of birds in which there are only 10 females. the numbers of viable offspring produced by each female this year were: 5, 3, 1, 2, 0, 6, 0, 0, 2, 5. what was the fecundity in this population this year?

Answers

Fecundity can be defined as the number of offspring produced by an individual or population.

In this case, we need to find the fecundity of the bird population given the number of viable offspring produced by each of the 10 females.

We can do this by adding up the number of viable offspring produced by all the females and then dividing by the number of females.

That is, Fecundity = (5 + 3 + 1 + 2 + 0 + 6 + 0 + 0 + 2 + 5) / 10

Fecundity = 24 / 10

Fecundity = 2.4

Therefore, the fecundity of this bird population this year was 2.4.

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please help: Which of the following molecules are products of glycolysis? Select all that apply, more than one answer.
A) 2 ADP molecules
B) 2 NADH molecules
C) 4 ATP molecules
D) 4 ADP molecules
E) 1 pyruvate molecule

Answers

Answer:

Glycolysis produces 2 ATP, 2 NADH, and 2 pyruvate molecules: Glycolysis, or the aerobic catabolic breakdown of glucose, produces energy in the form of ATP, NADH, and pyruvate, which itself enters the citric acid cycle to produce more energy.

Explanation:

Glycolysis starts with one molecule of glucose and ends with two pyruvate (pyruvic acid) molecules, a total of four ATP molecules, and two molecules of NADH. Two ATP molecules were used in the first half of the pathway to prepare the six-carbon ring for cleavage, so the cell has a net gain of two ATP molecules and 2 NADH molecules for its use. If the cell cannot catabolize the pyruvate molecules further (via the citric acid cycle or Krebs cycle), it will harvest only two ATP molecules from one molecule of glucose.

Mature mammalian red blood cells do not have mitochondria and are not capable of aerobic respiration, the process in which organisms convert energy in the presence of oxygen. Instead, glycolysis is their sole source of ATP. Therefore, if glycolysis is interrupted, the red blood cells lose their ability to maintain their sodium-potassium pumps, which require ATP to function, and eventually, they die. For example, since the second half of glycolysis (which produces the energy molecules) slows or stops in the absence of NAD+, when NAD+ is unavailable, red blood cells will be unable to produce a sufficient amount of ATP in order to survive.

Additionally, the last step in glycolysis will not occur if pyruvate kinase, the enzyme that catalyzes the formation of pyruvate, is not available in sufficient quantities. In this situation, the entire glycolysis pathway will continue to proceed, but only two ATP molecules will be made in the second half (instead of the usual four ATP molecules). Thus, pyruvate kinase is a rate-limiting enzyme for glycolysis.

Glycolysis produces 2 NADH, 2 ATP, and 2 Pyruvate molecules. Hence, option B is the correct answer.

Glycolysis is the process that is described as an oxidative pathway that typically breaks down the monosaccharides including galactose and glucose molecules and is further involved in the transformation of the energy released into chemical energy which can be used by cells. The products obtained from the glycolysis are:

2 ATP which is generated via substrate-level phosphorylation 2 NADH as a result of reaction named redox reactions 2 pyruvate molecules

Thus, we can conclude that the 2 NADH molecules from the option is the correct answer. Hence, option B is the correct answer.

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The equations represent two biochemical processes that occur in living organisms. The letter X represents a molecule produced from Process 1.
Process 1: oxygen + glucose → carbon dioxide + water + X
Process 2: carbon dioxide + water + X → oxygen + glucose

Q1. Which process occurs in the cells of a green plant leaf?
(1) Process 1, only
(2) Process 2, only
(3) Neither process 1 nor process 2
(4) Both process 1 and process 2

Q2. Which process occurs in the cells of an animal?
(1) Process 1, only
(2) Process 2, only
(3) Neither process 1 nor process 2
(4) Both process 1 and process 2

Answers

Answer:

help hurry someone anyone

Explanation:


What is meant by the following statement about the cell membrane?
The cell membrane is said to be semipermeable.

A. It does not contain any enzymes.

B. It allows only certain molecules to pass through.

c. It will not allow small molecules to pass through.

D. It allows all nonpolar molecules into the cell.

Answers

Answer:

The cell membrane is semipermeable because allows only certain molecules to pass through.

Explanation:

Semipermeability is a common property of cell membranes, due to their predominantly lipid composition, which gives them a hydrophobic character.

The cytoplasmic membrane is constituted by a double layer of lipids, in addition to structural proteins and glucides, which prevent the entry of polar or charged molecules. This characteristic allows lipids and small molecules without charge to pass freely through it.

Regarding the other options:

    A. Cell membranes are associated with lysosomes, structures that contain enzymes. However, this characteristic is not related to semipermeability.

    C. They allow the passage of small molecules - devoid of charge - through them.

    D. Not all non-polar molecules pass through, as some need a transporter.

Answer: B it only allows certain molecules to pass through

Explanation: permeability is the ability to let molecules pass through, and semipermeable only allow certain molecules through.

dogs do not get measles because their cells lack the correct receptor sites for that virus. dogs do not get measles because their cells lack the correct receptor sites for that virus. true false

Answers

The statement "dogs do not get measles because their cells lack the correct receptor sites for that virus" is true. Measles is a virus that affects humans and primates, but not dogs. The reason why dogs do not get measles is because their cells do not have the receptors that are necessary for the virus to enter and replicate within their bodies.

Receptor sites are specific areas on the surface of cells that allow viruses to bind and enter the cell, initiating the infection process. If a virus cannot find the right receptors on a cell, it cannot enter and replicate within that cell. In the case of measles, the virus can only infect humans and primates because their cells have the necessary receptors.

Dogs are not completely immune to all viruses, but they are affected by different types of viruses than humans. For example, dogs can get canine distemper virus, which is a highly contagious virus that can cause serious illness and death in dogs. However, canine distemper virus cannot infect humans because their cells lack the necessary receptors.

In conclusion, it is true that dogs do not get measles because their cells lack the correct receptor sites for that virus. This is due to the fact that dogs and humans have different types of cells with different surface receptors, which make them susceptible to different types of viruses.

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A student calculated the average surface area-to-volume ration of four different types of human epithelial cells.
Simple squamous: 9
Simple cuboidal: 6
Simple columnar: 4
Simple spherical: 3
Based on the data, which type of cell would be
best suited for the lining the alveoli of the lungs,
where diffusion of carbon dioxide and oxygen
must occur very rapidly?
a. Simple squamous cells
b. Simple columnar cells
c. Simple cuboidal cells
d. Simple spherical cells

Answers

The surface area-to-volume ratio of cells determines the rate at which molecules diffuse across the membrane. Simple squamous cells would be best suited for lining the alveoli of the lungs, where diffusion of carbon dioxide and oxygen must occur very rapidly, according to the data.  So, the correct option is a. Simple squamous cells.

The surface area-to-volume ratio of the simple squamous cell is 9. The ratio of surface area to volume is critical since it determines how fast molecules diffuse across the membrane. A high surface area-to-volume ratio allows for quick diffusion of gases such as oxygen and carbon dioxide, which are exchanged in the alveoli of the lungs.

Simple squamous cells are thin and flat, and their function is to facilitate diffusion. The cells in the walls of the alveoli, where gases are exchanged, are simple squamous cells.

Therefore, it is the simple squamous cells that would be best suited for lining the alveoli of the lungs where diffusion of carbon dioxide and oxygen must occur very rapidly.

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Where is DNA found in a eukaryotic cell?

Where is RNA found in a eukaryotic cell?

Answers

A) NucleusB) Cytoplasm                                HOPE IT HELPS (◕‿◕✿)                                                         SMILE!!

what are the parts that interact to form a whole ecosystem.

Answers

biotic factors and abiotic factors

20. Which of the following statements about binary fission is false?
a. In both prokaryotic and eukaryotic cells, the outcome of cell reproduction is a pair of daughter
cells, which are genetically identical to the parent cell.
b. Karyokinesis is unnecessary in prokaryotes because there is no nucleus.
c. Replication of the prokaryotic chromosome begins at the origin of replication and continues in
both directions at once.
d. The mitotic spindle draws the duplicated chromosomes to the opposite ends of the cell followed
by formation of a septum and two daughter cells.

Answers

Answer:

D. The mitotic spindle draws the duplicated chromosomes to the opposite ends of the cell followed by the formation of a septum and two daughter cells.

Explanation:

I took the quiz I got a 100%

The statement about binary fission 'the mitotic spindle draws the duplicated chromosomes to the opposite ends of the cell followed by the formation of a septum and two daughter cells' is FALSE.

Binary fission refers to an asexual mode of reproduction by which an organism separates a part of its body (usually a cell) to create two new bodies.

Binary fission represents the most common mode of asexual reproduction and occurs primarily in prokaryotes.

In binary fission, a parent cell divides to produce two genetically identical daughter cells.

During binary fission, there is no mitotic spindle formation within a cell nucleus.

In conclusion, the statement about binary fission 'the mitotic spindle draws the duplicated chromosomes to the opposite ends of the cell followed by the formation of a septum and two daughter cells' is FALSE.

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which describes the scientists who contributed to our current body of knowledge?
a. american women
b. men and women throughout time
c. Europeans in the 1800s
d. wealthy men of the last century

Answers

Wealthy men of the last century

5. The cell membrane is also called the _ membrane.​

Answers

Answer:plasma membrane

Explanation:

if an iron block is 7.9g and is cut into 4 pieces how much grams is each piece

Answers

Answer:

1.975g

Explanation:

If you cut the block into 4 equal pieces, then: 7.9÷4 = 1.975g

dural venous sinuses are specialized venous channels that run between the two layers of dura. they drain blood and cerebrospinal fluid from the brain. how do the dural venous sinuses differ from veins?

Answers

Dural venous sinuses differ from veins in various ways. Unlike veins, they lack the typical arrangement of three layers in their walls. This is due to the fact that they are covered by the meningeal layer of the dura mater, which provides them with an additional layer and increases the strength of their walls.

Additionally, they do not possess any valves like veins do, as their function is primarily passive. Because of their position, they do not need to return blood to the heart. They have thin walls, like veins, but are larger in diameter and can be tortuous in nature.

Furthermore, the flow of blood through dural venous sinuses is often slower than it is in veins because of their wide lumens. In contrast to veins, dural venous sinuses are also lined with endothelium, which is continuous with the meninges, and do not have smooth muscle or elastin fibers, which help to maintain venous blood pressure.

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which formed elements release vasoconstrictors, promote blood clotting, initiate dissolution of clots, and secrete growth factors? multiple choice question. platelets neutrophils basophils erythrocytes

Answers

Platelets are responsible for releasing vasoconstrictors, promoting blood clotting, initiating the dissolution of clots, and secreting growth factors. These functions are vital for maintaining hemostasis, wound healing, and tissue repair in the body.

Platelets are small, disc-shaped cellular fragments found in the blood. They play a crucial role in hemostasis, which is the process of stopping bleeding and maintaining the integrity of blood vessels. Platelets are responsible for various functions related to clotting and wound healing.Platelets release vasoconstrictors: When blood vessels are damaged, platelets adhere to the site of injury and release vasoconstrictors such as serotonin and thromboxane A2. These substances cause the blood vessels to constrict, reducing blood flow to the injured area and minimizing blood loss.Platelets promote blood clotting: Platelets contain specific proteins and factors that are essential for the formation of blood clots. When platelets come into contact with exposed collagen fibers at the site of injury, they become activated and change shape. This activation triggers a cascade of reactions that lead to the formation of a blood clot, known as a platelet plug.Platelets initiate dissolution of clots: Once a clot has fulfilled its role in stopping bleeding and the injured blood vessel has healed, the body needs to dissolve the clot to restore normal blood flow. Platelets release substances, such as plasminogen activators, which activate the enzyme plasmin. Plasmin then breaks down the fibrin network that holds the clot together, leading to clot dissolution or lysis.Platelets secrete growth factors: Platelets contain various growth factors, such as platelet-derived growth factor (PDGF) and transforming growth factor-beta (TGF-beta). These growth factors are released from platelets and play a crucial role in tissue repair and wound healing. They stimulate cell division, promote the growth of new blood vessels (angiogenesis), and aid in the regeneration of damaged tissues.

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why the carbon cycle is important to the environment?

Answers

Answer:The carbon cycle is important in ecosystems because it moves carbon

Explanation: a life-sustaining element, from the atmosphere and oceans into organisms and back again to the atmosphere and oceans. ... Scientists are currently looking into ways in which humans can use other, non-carbon containing fuels for energy.

themes in science include

Answers

Answer:

cycles, equilibrium, change, models, scientific method

Explanation:

Answer:

Ocean-Atmosphere Exchange
Climate Variability, Ocean Circulation, and Ecosystems
Turbulent Mixing and Biophysical Interactions

What is a source of sediment?


fragments of other rocks


chemicals and minerals dissolved in water


sea shells


all of the above

Answers

Answer:

all of the above is the right answer

Yee all of the above

CHAPTER: Conservation of Plants and Animals

Fill in the blanks:
__________ species dont occur anymore​

PĹŚ ĤĔĹP

Answers

Answer:

Extinct

Explanation:

Which protostome phyla successfully made the transition from water to land?
a) Mollusca
b) Arthropoda
c) Annelida
d) Nematoda
e) all of the above

Answers

Arthropoda protostome phyla successfully made the transition from water to land. The correct answer is option (b).

Arthropoda is the protostome phylum that successfully made the transition from water to land. It comprises over 80% of all known animals, including insects, spiders, and crustaceans. Members of this phylum share several features, including a hard exoskeleton, segmented bodies, and jointed appendages. As a result, they are capable of moving, sensing their environment, and feeding in ways that were impossible for their softer-bodied ancestors.

Arthropods are the most successful of the phyla that made the transition from water to land. Their exoskeleton is a major reason for their success, as it helps to prevent water loss and provides a rigid framework for muscle attachment. Insects, for example, have evolved spiracles, tiny openings in their exoskeleton, that allow air to enter and exit their body. Insects and some crustaceans have evolved wings, which give them the ability to fly and access new food sources.

Arthropods have also adapted to terrestrial environments in other ways. Some have evolved a waxy coating to prevent water loss, while others have evolved compound eyes to better see their surroundings. Finally, some arthropods, like scorpions and spiders, have evolved venomous stingers or fangs to protect themselves from predators.

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All cells are small in size. Which of the following is the smallest cell?

Answers

Answer:

Mycoplasma

Explanation:

Mycoplasma is the smallest cell

what is the step of the scientific method that involves test of hypothesis

Answers

Answer:

1) Make an observation that describes a problem,

2) Create a hypothesis,

3) Test the hypothesis

4) Draw conclusions and refine the hypothesis.

The basic steps of the scientific method are: 1) make an observation that describes a problem, 2) create a hypothesis, 3) test the hypothesis, and 4) draw conclusions and refine the hypothesis.

which of the following processes is not common during the post-absorptive state?
-glucose catabolism
-ketogenesis
-protein catabolism
-glycogenolysis

Answers

The process that is not common during the post-absorptive state is glucose catabolism.

The post-absorptive state, which is also known as the fasting state, refers to the period after food consumption has ceased. During the post-absorptive state, energy must be derived from stored macronutrients in order to maintain blood glucose levels and support other physiological processes. Glucose catabolism is a process in which glucose is oxidized in order to produce energy, which is utilized to carry out cellular processes.

This process typically occurs in the presence of oxygen, and is known as aerobic respiration. In the post-absorptive state, glucose levels decrease and the body relies on alternative sources of energy, such as fatty acids and ketone bodies, which are produced via ketogenesis. Thus, glucose catabolism is not common during the post-absorptive state as glucose levels are low and the body must rely on other sources of energy.

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What is the major difference between a bar graph and a line graph

Answers

Line graphs are used to display data or information that changes continuously over time. Line graphs allow us to see overall trends such as an increase or decrease in data over time. Bar graphs are used to compare facts. The bars provide a visual display for comparing quantities in different categories or groups.
line graphs display information that changes continuously, bar graphs display comparisons to different quantities!

Then sort the statements according to whether they are strengths or weaknesses

Answers

Which statements

Ignore
Hey hey hey hey

What two things make up a solution

Answers

Answer:

thinking positive and solve generally

In the lungs,the blood gives up_____ and picks up _______.

A: Carbon dioxide,nutrients

B: nutrients,oxegyn

C: oxegyn,carbon dioxide

D: carbon dioxide,oxegyn


PLEASE TELL ME FILL IN THE BLANKS

Answers

Answer:

carbon dioxide,oxegyn

Explanation:

in the lungs,the blood gives up__carbodioxide___ and picks up ____oxygen___.

A research participant hears a tone, which is followed by a puff of air directed toward his eye. Later, he blinks when he hears the tone. Before ending the experiment, what could the researcher do in order to extinguish the blinking to that tone?
Group of answer choices

Repeatedly present the tone alone

Repeatedly present the puff of air alone

Increase the loudness of the tone

Increase the amount of air that is directed toward the eye

Answers

Answer:

Repeatedly present the tone alone

Explanation:

In the context above, It was said that he blinks when he hears the tone. Before ending the experiment, it was deliberated upon what the researcher would do in order to extinguish the blinking to that tone.

The best thing to do will be to repeatedly presss the tone alone so he gets used to it and reacts differently.

Perception Which of the following refers to the transformation of stimulus energy into neural impulses?
A) Perception
B) Transduction
C) Bottom-up processing
D) Top-down processing
E) Psychophysics

Answers

Perception refers to the process of transforming stimulus energy into neural impulses. It is a complex process that involves a variety of steps.

Correct option is A.

This includes transduction, the process by which physical energy is converted into neural energy, and both bottom-up and top-down processing, the cognitive and perceptual processes by which information is input and used to interpret an environment. Bottom-up processing relies on sensory details taken from the environment, while top-down processing relies on past experiences and expectations to interpret sensory details.

This process is also underpinned by psychophysics, which is the study of how physical signals are perceived by an organism. Therefore, all of the steps involved in the transformation of stimulus energy into neural impulses are encompassed within the concept of perception.

Correct option is A.

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Onepage review on why understanding gas and vapors in a work setting is important and what we can do in our workplace to prevent accidents with gases and vapors.

Answers

Understanding gas and vapours in a work setting is incredibly important for workplace safety. Gas and vapours can contain noxious substances that, if exposed to, can lead to serious injury or health implications.

In order to prevent such accidents, employers must perform risk assessments in order to assess and identify any areas of risk and put in place control measures to limit them. If gas and vapours are used onsite, then suitable ventilation and extraction systems should be in place.

Employers should also ensure that their staff are aware of the potential risks associated with such substances and provide adequate training and instruction in how to work safely. Finally, employers should also check that any necessary personal protective equipment is provided and communications are in place to be able to notify relevant emergancy responders in case of an accident.

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Explain why there are many first order consumers in the prairie food chain?

-The prairie is a place where many herbivores live due to the abundant amount of food source.
-There are not many decomposers found in the prairie.
-The prairie has more space for herbivores to live in.
-The prairie provides more sunlight and water for herbivores to live in.

Answers

Answer: the prairie is a place where many herbivores live

Explanation:

I got it right

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A company opened on January 1 of the current year. During January, the following transactions occurred and were recorded in the company's books: 1. The company received $14,000 cash for services provided. 2. The company paid $2,600 cash for an insurance policy covering the next 24 months. 3. The company received $6,200 cash for services provided. 4. The company purchased $6,700 of office equipment on credit. 5. The company provided $3,250 of services to customers on account. 6. The company paid cash of $2,000 for monthly rent. 7. The company paid $3,600 on the office equipment purchased in transaction #5 above. 8. Paid $325 cash for January utilities. Based on this information, the balance in the cash account at the end of January would be: Multiple Choice $47,950. $11,675 $20,200. $16,250. $14,925. 4. 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