which of the following methods could be used to detect the presense of viable but nonculturalbe celss of a pathogen in a water or food specimen? a) culture on enriched agar; b) use of enrichment techniques; c) polymerase chain reactions; d) culture on selective agar

Answers

Answer 1

The following techniques are used to find DNA: Analysis of one UV- Vis Spectrophotometric Fluorometric evaluation. Precipitation of DNA Electrophoresis of DNA gel Polymerase chain reaction.

As a result, even with a tiny amount of sample (such as one taken from a crime scene), we may still detect the presence of DNA. DNA might be present in our sample if the 260/280 ratio was between 1.77 and 1.88.

Rapid microbial methods (RMMs), also known as alternative microbiological methods or alternative microbiology test methods, are technologies that allow users to get results from microbiology tests more quickly than with traditional culture-plate procedures. Miller: "Faster" refers to in a matter of hours as opposed to, in some circumstances, days or weeks.

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Related Questions

Explain how precipitation affects the phosphorus cycle

Answers

When precipitation increases that increases erosion and will also increase the transport of phosphorous and other nutrients into soils, environments, and fresh water. Habitat loss and erosion can reduce the nutrient in soils.

Which atom attains a stable valence electron configuration by bonding with another atom?.

Answers

Hydrogen atom attains a stable valence electron configuration by bonding with another atom.

Hydrogen atom is chemically stable as they share an electron with another hydrogen atom. When Combined with a hydrogen molecule consisting of two atoms with one oxygen atom they make a compound known as water.

They are capable of achieving a stable configuration by filling up the outer shell by donating or sharing one electron with another atom or a molecule such as water. Hydrogen atoms combined together to form a covalent bond because they share their electrons equally to form a hydrogen molecule.  

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When a steroid hormone binds to an intracellular receptor, what happens after the receptor changes shape?.

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When the steroid hormone binds to an intracellular receptor, the receptor shape changes and that leads to activation of the process of transcription.

Steroid hormones are responsible for regulating cellular processes and they do this by binding to intracellular receptors. These intracellular receptors interact with the nucleotide sequences to alter gene expression.

Most steroid receptors target cells are located in the cytoplasm, they get to the nucleus to regulate the gene expression. Here, the steroid hormone comes into play. It crosses the membrane of the target cell to enter into the cytoplasm where it binds to a specific receptor made of protein.

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Selenium is a trace mineral that is part of the structure of certain proteins. One of these proteins is an antioxidant enzyme that neutralizes peroxides before they can form free radicals. What is the name of this protein?.

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Selenium (Se) is a crucial trace element, and studies have shown that those who have low levels of it have an increased risk of developing illnesses including cancer and heart disease. proteins (selenoproteins) or enzymes containing se (selenoenzymes) are antioxidants.

Selenoproteins and enzymes contain selenium as a antioxidants . These are antioxidants and aid in dissolving peroxides, which can harm DNA and tissues and cause inflammation and other health difficulties. By controlling the amount of free radicals, antioxidants like selenium assist in reducing oxidative stress. They function by scavenging surplus free radicals and shielding cells from oxidative stress-related harm.

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The region of a drosophila embryo with the highest concentration of bicoid protein will develop into the _____.

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The maternal bicoid (bcd) gene orchestrates earlier development in Drosophila. Its mRNA is located at the anterior tip of the oocyte and early embryo.

bicoid is expressed in the anterior region of the egg, where it plays its role in the anterior body patterning of the fly larva. The Bcd concentration gradient is generated from mRNA produced by nurse cells and transported to the oocyte where it is sequestered in an inactive state at the leading end (Frigerio et al., 1986).

Bicoid mRNA localizes to the Drosophila anterior oocyte from stage 9 of oogenesis onwards to provide a local source of bicoid protein for embryonic patterning. in an inactive state at the leading end (Frigerio et al., 1986).

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using the appropriate atp equivalents for nadh and fadh2, how much total atp (and/or atp equivalents) would the complete oxidation of 3-phosphoglycerate yield?

Answers

The complete oxidation of 3-phosphoglycerate would yield 20 ATP.

How many ATP's are gained by complete oxidation of phosphoglyceric acid?

During glycolysis, each phospho-glyceric acid yields one pyruvic acid. Each pyruvic acid is transformed into acetyl CoA, which produces a NADH. The Krebs cycle results in the full oxidation of acetyl CoA. Three NADH, one FADH[tex]_{2\\[/tex], and one GTP are the net gains of high-energy molecules from one cycle; the GTP can be used to make ATP later on. The total number of ATP molecules created is 3 + 3 + 3 + 3 + 2 + 1 = 21 ATP since each NADH is comparable to 03 ATP and each FADH[tex]_2[/tex] is equivalent to 02 ATP.

Additionally, 1 ATP is required in the process of converting phosphoenolpyruvate to pyruvic acid, resulting in a net gain of 20 ATP.

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What are the 4 elements of a memoir?.

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A theme ,Obstacles,Emotional beats,Personal style are four elements of memoir.

Simple: Start at the beginning of your life and end about the time you made the decision to write your autobiography memoir.

Although it makes sense to begin with your birth, you could wish to begin by briefly reviewing your family's history.

You can also decide to skip your formative years and start later in life.

Your life biography should follow a chronological order, although it is not required to list your years of existence in detail.project future events. Third are:your outlook on your future, how you envision your life unfolding, and a forecast of where your life will go through your own eyes.

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What is the top of a hierarchy called?.

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The highest-ranking people are at the apex.

The most common way to represent a hierarchy is as a pyramid, where the width of the level represents how many individuals or business divisions are there in relation to the whole, and the height of the ranking or person depicts their power status.

The highest-ranking individuals are located at the apex and there are typically very few of them, often only one; the base may contain thousands of individuals who have no subordinates.

An organizational chart or organogram is created by using a tree or triangle diagram to represent these hierarchies. Because there are fewer people at the top than at the bottom, those closest to the top have more influence than those closest to the bottom.

As a result, in a hierarchy, superiors typically command and have a greater rank.

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Deep sea ocean ecosystems in the region of hydrothermal vents typically have much higher biomass with greater presence of larger eukaryotic organisms than other deep sea ecosystems. Which of the following is most responsible for this difference? A.) temperature differences B.) greater O2 content in regions around hydrothermal vents C.) pressure differences D.)much greater numbers of photosynthetic organisms E.) presence of substantial amounts of highly reduced inorganic compounds and methane

Answers

Deep-sea ocean ecosystems in the region of hydrothermal vents typically have much higher biomass with a greater presence of larger eukaryotic organisms than other deep-sea ecosystems due to the presence of substantial amounts of highly reduced inorganic compounds and methane (Option E).

What are deep-sea ecosystems?

The expression deep-sea ecosystem is used to denote certain biomes which are relatively independent of the level of sunlight energy provided by the sun and the presence of photosynthetic species.

Therefore, with this data, we can see that deep-sea ecosystems are able to survive in absence of sunlight and therefore the biomass in these ecosystems is associated with the presence of highly reduced inorganic compounds as well as methane.

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The purpose of _______________ is making more cells to repair wounds and replace dead cells, for asexual reproduction of single-celled eukaryotes, and for growth during development.
the mitotic cell cycle

Answers

The mitotic cell cycle.

What is mitotic cell cycle?

The body creates new cells to replace damaged or dying cells. Cell division is the name given to this process. One cell divides into two to become two cells. Four cells are created from two, and so on.

Even after a being reaches adulthood, tissue regeneration or repair still requires cell proliferation. For instance, fresh skin and blood cells are constantly being created. Cell division is a fundamental process in the growth, maintenance, and repair of cells and tissues in all multicellular animals.

Similar to how a blood clot prevents additional blood loss, the process of wound healing inhibits further material loss. The process by which the cell selectively recreates and replaces the missing parts (organelles, plasma membrane, cytoplasm, etc.) 

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What is true about a mineral with a hardness of 5 on the Mohs Hardness Scale?


Question 19 options:


A.) it can be scratched by an iron nail, but not by glass



B.) it can be scratched by glass, but not by an iron nail



C.) it can't be scratched by anything

Answers

Mineral with the hardness of 5 on the Mohs Hardness Scale it can be then scratched by the iron nail, but not by glass

Diamond is the hardest mineral, so it is always at the top of the scale. His 10 minerals are Mohsscale, talc, gypsum, calcite, fluorite, apatite, feldspar, quartz, topaz, corundum and finally the hardest diamond. The Mohs hardness scale is used as a convenient method of identifying minerals. Mineral hardness is a measure of relative scratch resistance, measured by scratching a mineral against another substance of known hardness on the Mohs scale. Minerals are the naturally occurring the  inorganic elements or the compounds with the ordered internal structures and the characteristic chemical compositions, the crystal forms, and the physical properties. Common then minerals include the quartz, feldspar, mica, the amphibole, olivine, and calcite.

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natural light causes the flowers to bloom necessary sufficient necessary and sufficient contributory

Answers

Flowers bloom because of natural light, which is essential, sufficient, and necessary.

The answer is (d), The answer is (d) , The answer is (c), The answer is (b)

1) The answer, which is (d), is a contributing cause. While undoubtedly crucial, sunshine is not the only component that leads to flowers blooming. Numerous indoor plants may produce blossoms even in the absence of sunshine. Therefore, it is a contributing factor, and the solution is (d).

2) The response, which is (d), is a contributory cause. Aggression can be triggered by violent video games, but it is not the only reason. A genetic disease may potentially contribute to aggression, among many other possible causes. Although it influences aggressiveness, it may not always cause hostility. Consequently, it is a contributing factor, and the solution is (d).

3) The appropriate response is (c), which is a sufficient and essential cause. Paper may burn if it is placed in an incinerator, as combustion or heat is a crucial and required element in burning. Without heat or combustion, paper cannot be burnt. Due to its adequate and required nature, the solution is (c).

4) The solution, which is a sufficient cause, is (b). It is true that smoking is damaging to our health and dangerous. The risk of lung cancer is also present. It is an adequate cause of lung cancer. Approximately 80% of persons with lung cancer had a history of smoking. The conclusion is that it is a sufficient reason (b).

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it can be assumed that a ribosome met a blank in a(n) blank and blank stopped resulting in a shortened protein. in human cells, blank are removed by blank before translation starts, but there is no blank in bacterial cells. also, some part of amino acids in the resulting protein was blank because they had been translated from blank. this fact could cause the production of a nonfunctional protein.

Answers

It can be assumed that a ribosome met a stop codon in a(n) intron(s) and translation stopped resulting in a shortened protein. In human cells, introns are removed by RNA splicing before translation starts, but there is no RNA splicing in bacterial cells. Also, some part of the amino acids in the resulting protein was incorrect because they had been translated from introns. This fact could cause the production of a nonfunctional protein.

Ribosomes are macromolecular machines, establish inside all containers, that act as biological protein combinations. Ribosomes link amino acids together in the order particularized for one codon of prophet RNA fragments to form polypeptide chains. Ribosomes amount to two important components: the limited and abundant ribosomal subunits.

In microscopic biology and genetics, translation is the process at which point ribosomes in the cytoplasm or endoplasmic web synthesize proteins afterward the process of a copy of DNA to RNA in the container's core. The whole process is named deoxyribonucleic acid expression.

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according to the epa water contaminant chart, two contaminants that are sourced from human and animal fecal matter are:

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Two contaminants originating from human and animal feces are fecal coliform and E. coli.

The presence of contaminants in drinking water is normal, water sources or drinking water treatment processes may contain or produce contaminants in water. The presence of contaminants does not necessarily indicate that the drinking water is hazardous. While some types of drinking water contaminants may not be harmful, others may pose a small health risk if consumed.

Water contaminants can be classified into 3 groups, namely physical, chemical and biological contaminants.

Physical contaminants include odor, color, taste, turbidity, temperature, suspended solids, colloids, and dissolved materials.Chemical contaminants include organic matter (BOD, COD, TOC, KMn04), nitrogen compounds, metals (Fe, Mn, Mg, Cu, Cr, Pb, Hg, Cd), and hardness.Biological contaminants include various types of bacteria or viruses.

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cervical plexus - serves the head, neck and shoulders. brachial plexus - serves the chest, shoulders, arms and hands. lumbosacral plexus. lumbar plexus - serves the back, abdomen, groin, thighs, knees, and calves. ... coccygeal plexus - serves a small region over the coccyx.

Answers

The spinal plexus includes: Cervical Plexus, Brachial Plexus, Lumbar Plexus, Sacral Plexus, Coccygeal Plexus.

What is the spinal plexus?

The term plexus refers to the "web" of nerves that arises immediately adjacent to where T12-L5 exits the spinal cord through the intervertebral foramen. Plexuses are present on both sides and allow nerves to connect at different levels to create multiple functioning nerves that serve to innervate different structures. It includes the following regions:

The cervical plexus serves the head, neck and shouldersThe brachial plexus nourishes the chest, shoulders, arms and handsThe lumbar plexus supplies the back, abdomen, groin, thighs, knees, and calvesThe sacral plexus serves the pelvis, buttocks, genitals, thighs, calves, and feetThe coccygeal plexus serves a small area above the tailbone

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Complete question is as follows:

Describe the five regions of the spinal cord and the associated nerve plexuses.

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If a person were born with only green and blue cone cells, which color would the
person have trouble seeing? How might this condition affect the person?

Answers

If a person only has blue and green cone cells, he/she has red colorblindness, or the inability to see red as well as other color spectra of red such as orange.

What do you mean by colorblindness?

Color blindness occurs when you are unable to see colors in a normal way. It is also known as color deficiency. Color blindness often happens when someone cannot distinguish between certain colors. This usually happens between greens and reds, and occasionally blues.

If you have color blindness, it means you see colors differently than most people. Most of the time, color blindness makes it hard to tell the difference between certain colors. Usually, color blindness runs in families. There's no cure, but special glasses and contact lenses can help.

The most common kinds of color blindness are genetic, meaning they're passed down from parents. If your color blindness is genetic, your color vision will not get any better or worse over time.

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How many o2 molecules are there?.

Answers

One molecule of O2 molecule is having a mass of 32 g contains the 6.022 X 10 raise to the power of 23 molecules of oxygen. Each atom has two molecules of oxygen.

What is the atomic number of  oxygen ?

The atomic number of oxygen is 16.

In each of O2 molecule there is a presence of 2 oxygen atoms that is equivalent to the Avogadro number that is equal to the number mentioned above where the molecules is the total number of molecules that are present in the molecule.

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(fill in the blank) is the smooth endoplasmic reticulum of muscle fiber that contains terminal cisternae where calcium is stored

Answers

The smooth endoplasmic reticulum of muscle fibers has terminal cisternae, which is where calcium is stored.

What is the name of the muscle fiber's smooth ER?

Sarcoplasmic reticulum, a specific membrane structure, is where SER is found in skeletal muscle cells. In order to store calcium ions, the sarcoplasmic reticulum absorbs the ions from the cytoplasm.

Which organ stores calcium: Ser or RER?

Within cells, the SER serves as a calcium storage area. Active transport pumps calcium into the SER, where it is released in response to hormonal cues. This is crucial because the SER is so prominent in muscle cells that it goes by the name of sarcoplasmic reticulum.

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What caused Hamlet's tragic end?.

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Unreality In William Shakespeare's Hamlet. even though Hamlet manages to correctly kill his uncle and avenge his father, he dies because Laertes swipes him with his poisoned sword; his revenge leads to his demise.

Hamlet's tragic flaw became his hesitancy to act. whilst many readers would possibly think his insanity is his flaw, his hesitancy to take action creates the maximum trouble in the play. whilst Claudius believes Hamlet may understand the truth, as an example, he has time to make plans at the same time as Hamlet agonizes approximately what to do.

Shakespeare's tragic hero Hamlet's deadly flaw is his failure to behave right now to kill Claudius, his uncle, and the murderer of his father. His tragic flaw is 'procrastination'. His continuous recognition and doubt delay him in performing his wishes.

Ophelia is by way of far one of the most tragic characters in Shakespeare's play The Tragedy of Hamlet. She loses the whole thing she holds valuable, inclusive of her father and Hamlet's affections, and is manipulated by the men who declare to love her.

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Did Hamlet and Ophelia sleep together?.

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It would have been hazardous for Shakespeare specifically to depict pre-marital sex between noble characters, but Hamlet gives us reasons to suspect that at a few points sometime recently the start of the play, Hamlet and Ophelia have had sex.

Laertes and Polonius both caution Ophelia against having sex with Hamlet, which recommends that Ophelia’s father and brother, at least, are concerned about the possibility. Afterward within the play Hamlet teases Ophelia with expressly sexual plays on words, assist proposing that they may have shared intimacy. For occurrence, fair some time recently in the play scene, he inquires: “Shall I lie in your lap, my lady?".the best proof that Hamlet and Ophelia have had sex comes from Ophelia. When Hamlet murders Ophelia’s father, she goes mad. In her madness, she sings melodies that appear to stay on the causes of her grief. A few of her tunes are approximately old men or fathers passing on. The rest are approximately pre-marital sex.

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Colonoscopy is the examination of the large colon. It allows for visual diagnosis of ulcers and polyps, which may lead to colon cancer. A polyp _____.is a cluster of abnormal cells

Answers

Colon cancer-causing ulcers and polyps can now be visually diagnosed. A collection of abnormal cells is known as a polyp carcinogen.

Explain about the polyp carcinogen?

If given the chance to develop over time, these polyps could develop into cancer. The term adenomas refers to the majority of these colon polyps. The size and location of the serrated polyps in the colon will determine whether they develop into cancer. Generally speaking, neoplastic polyps have the highest risk of malignancy among all polyp sizes.

A tissue growth that protrudes from the colon or rectum is referred to as a colorectal polyp. Although benign (i.e., not cancerous) and non-proliferating, some forms of polyps have the potential to turn malignant over time. The term "adenomatous polyps" refers to one of these varieties. We refer to these as pre-cancerous polyps.

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ballast water carried by ships is released near ports. why has this activity increased the potential for non-native aquatic species invasions?

Answers

Ballast water carried by ships is released near ports. Why has this activity increased the potential for invasions by non-native aquatic species? The regional species pool of non-native aquatic species has increased.

If organisms within a ship's ballast tanks survive the voyage to the next destination, they may be released with the ballast water into waters where they do not occur naturally. If these non-native organisms survive and spread throughout their new environment, they can become invasive species. Ballast water dispersal of invasive species refers to the unintentional introduction of invasive species into new habitats via ballast water carried by commercial vessels. Ballast water spreads some 7,000 living species to new habitats around the world.

Ships routinely take in ballast water to ensure stability and structural integrity. It can contain thousands of aquatic microbes, algae, and animals, which are then transported across the world's oceans and released into ecosystems where they are not native.

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Which learning process occurs when the same response happens in reaction to many similar stimuli?.

Answers

Generalization is the learning process that occurs when the same response occurs to many similar stimuli.

What is generalization?

Generalization is the concept that humans and other animals use previous learning in a current learning situation, where the conditions of the situation are considered similar. Generalizations include three specific forms:

Stimulus generalizationResponse generalizationMaintenance.

Stimulus generalization is the ability to act in new situations in ways learned in other similar situations. After being conditioned to respond to a particular stimulus, it increases response to other stimulus situations.

Stimulus generalization occurs when an individual responds to a stimulus similar to the original conditioned stimulus. For example, dogs trained to drool when they hear a click may generalize this behavior to sounds similar to knocks and beeps.

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Multicellular organisms generally utilize more complex gene regulation than unicellular organisms via mechanisms that include?.

Answers

Multicellular organisms generally utilize more complex gene regulation than unicellular organisms via mechanisms that include transcriptional repression and epigenetic modification.

The correct option is E.

What is gene?

The most fundamental component of heredity, the gene contains the genetic code for every feature and characteristic. There are two alleles in each gene. On the chromosomes, these genes can be found.

DNA fragments called genes typically contain the instructions needed to produce a particular protein.

Why are genes important?

Genes include instructions that explain to your cells how to function and develop. The building blocks of the body are cells. There are billions of cells functioning together in every part of your body. Chromosomes are the units that house genes.

What is transcriptional repression?

An important mechanism for the precise regulation of gene expression is transcriptional repression. They are typically thought of as proteins that attach to promoters in a way that prevents RNA polymerase from adhering afterwards.

What is epigenetic modification?

The non-heritable changes to genes are referred to as epigenetic modification. This modification does not mess with the genes' sequence. This means that they cannot be passed down from one generation to the next. The expression of genes can be controlled by these alterations, though. DNA methylation, histone modification, and noncoding RNA action are the three different types of genetic alterations.

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I understand that the question you are looking for is:

Multicellular organisms generally utilize more complex gene regulation than unicellular organisms via mechanisms that include?

A) transcriptional repression

B) polycistronic RNAs

C) epigenetic modification

D) polycistronic RNAs and transcriptional repression

E) transcriptional repression and epigenetic modification

What body composition measurement method uses low-intensity x-rays to scan the body and can also determine bone mineral density?.

Answers

The body composition measurement method which uses low-intensity x-rays to scan the body and can also determine bone mineral density is referred to as DEXA scan.

What is DEXA scan?

This is also known as dual-energy X-ray absorptiometry and it is referred to as a type of medical imaging test which is used to view the various structure and parts of organisms present or available.

Its mechanism of action is that it uses very low levels of x-rays to measure how dense objects are such as the bones etc. It employs the use of spectral imaging and it is commonly used for the diagnosis of osteoporosis.

This is done by aiming at the bone of the patient to determine its strength and know the type of treatment to be given thereby making it the correct choice.

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Molten gold is poured into moulds to make solid gold bars. What is the name of this process of a liquid becoming a solid?.

Answers

Answer:

Freezing

Explanation:

Freezing is when a liquid turns into a solid due to the temperature being lowered.

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Which of the following is a reason why ADHD was not included when the federal government began tracking the prevalence of disabilities?A) There was a large body of research suggesting it is not a distinct disability.B) The advocacy base for children with ADHD was not yet well-developed.C) There was already a category for minimal brain injury.D) The federal government put a cap on the number of disability types to be served by public schools.

Answers

When the federal government started documenting the prevalence of disabilities, ADHD was not included since the advocacy base for children with ADHD was not yet well-developed.

What subset does ADHD fall into?

However, rather than being classified as a "Specific Learning Disability," ADHD is classified as a "Other Health Impaired." If their ADHD has a significant negative impact on a vital life function like learning, individuals with ADHD may also be eligible for accommodations under the ADA and Section 504.

Why isn't ADHD seen as a disability?

Only when ADHD interferes with a person's capacity to work and participate in society is it considered a protected impairment; mild cases that don't affect functionality are not included. According to the Centers for Disease Control, ADHD is a developmental disorder.

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The hiv virus attacks only a certain type of white blood cells, and not other cell types. Why?.

Answers

the hiv virus does attack othernorgans n cells n the body especially the arteries and the heart

looking at the cell of a photosynthetic microorganism, you observe that the chloroplasts are green in brightfield microscopy and red in fluorescence microscopy. You conclude that
a. chlorophyll is fluorescent b. the magnification has distorted the image c. you're not looking at the same structure in both microscopes d. the stain masked the green color e. none of the above

Answers

As chlorophyll is fluorescent, you can see that the chloroplasts are red in fluorescence microscopy and green in brightfield microscopy.

What shade fluoresces in chlorophyll?

When exposed by light of around 400-700 nm (photosynthetically active radiation, or PAR), photosynthetic tissues and organisms emit red to far-red light, which is known as fluorescence (McCree 1972). In this spectrum, red and blue light are more effective at stimulating chlorophyll than green light.

What is a study of chlorophyll fluorescence?

Chlorophyll fluorescence is a frequent method in plant physiology for measuring photosystem II (PSII) activity since it is non-invasive. One of the most effective and popular methods for examining how pressures affect the photosynthetic process is chlorophyll fluorescence analysis.

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penicillin is an antibiotic that kills bacteria by binding to the active site of an enzyme involved in synthesis of the bacterial cell wall. which of the following phenomena best describes the mechanism of action of penicillin?

Answers

A phenomenon which best describes the mechanism of action of penicillin is: B. competitive inhibition.

What is competitive inhibition?

In Science, competitive inhibition can be defined as a phenomenon that typically occurs when molecules or chemical substances that are very similar to the substrate molecules are bounded to the active site of an enzyme, thereby, inhibiting (preventing) the binding of the actual substrate.

What is a penicillin?

A penicillin can be defined as an antibiotic that is designed and developed to kill (eliminate) bacteria, especially by binding to the active site of an enzyme that is involved in producing or constructing the bacterial cell wall.

In this context, we can reasonably infer and logically deduce that penicillin is an example of a competitive inhibitor because it blocks the active site of an enzyme that is used by several bacteria in the synthesis of their cell wall.

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Complete Question:

Penicillin is an antibiotic that kills bacteria by binding to the active site of an enzyme involved in synthesis of the bacterial cell wall. which of the following phenomena best describes the mechanism of action of penicillin?

noncompetitive inhibition

competitive inhibition

allosteric regulation

feedback inhibition

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